葡萄试管苗土支撑培养带坨移栽研究

以土作为培养基支撑物，离体培养葡萄试管苗双节茎段，４０天后即达到适于炼苗标准，成苗率９５．８％。带坨移入营养钵，在不喷雾，不覆膜，空气湿度低至４５％条件下，成活率达９６．７％。成活的试管苗炼苗５４天后移入大田，移栽成活率为１００％，其中特级苗占８７．５％，一级苗占１２．５％。试验证明，土支撑培养带坨移栽技术是简化试管苗移栽程序，降低成本的有效措施。

A Study on Transplanting with Lump Grape Plantlets in vitro Cultured in Soil Supporting Mdium

Stem sections with two nodes from in vitro cultured grape plantlets were cultured in soil supporting medium for 40 days, 95.8% of the stem sections produced new plantlets which subsequently underwent a four-week-long acclimatization. The ptantlets were then transplanted with lump into soil, and 96.7% of them survived under the conditions of no spraying of water,no plastic film covering, and a low relative air humidity of 45%. 54 days later, the survived plantlets were further transported into field, and in autumn they all formed young plants in which the super-grade plants and first-grade plants accounted for 87.5% and 12.5%respectively. The results showed that the techniques described above can effectively simplify the transplant procedure and reduce the cost of the plantlets derived from in vitro culture.