|
|||||
|
|
| 超声波处理对苹果花粉介导转基因的影响 | |
| 引用本文： | 曹秋芬,岳新丽,孟玉平,季兰,樊新萍.超声波处理对苹果花粉介导转基因的影响[J].华北农学报,2005,20(2):16-18. |
| 作者姓名： | 曹秋芬 岳新丽 孟玉平 季兰 樊新萍 |
| 作者单位： | 1. 山西省农业生物技术研究中心,山西,太原,030031 2. 山西农业大学,园艺学院,山西,太谷,030801 3. 山西省农业科学院,果树研究所,山西,太谷,030815 |
| 基金项目： | 山西省科技攻关项目(021034)，山西省留学回国人员资助项目(2001-15-75) |
| 摘 要： | 为了探讨花粉介导转基因过程中附加超声波处理对苹果品种丹霞和嘎拉花粉的影响，对苹果花粉处理的缓冲液和超声波的各参数进行了选择，设置了不同的缓冲液pH值、蔗糖浓度；超声波处理功率、时间、次数等。结果表明：适合苹果花粉处理的缓冲液蔗糖浓度为10％，pH值为5．4；超声波处理功率、时间、次数等各参数对苹果花粉的萌发率和破碎率有显著影响。 |
| 关 键 词： | 超声波处理 花粉介导 转基因 花粉处理 蔗糖浓度 缓冲液 苹果品种 苹果花粉 pH值 破碎率 萌发率 功率 时间 |
| 文章编号： | 1000-7091(2005)02-0016-03 |
Effects of Ultrasonication Treatment on Genetic Transformation by Pollen-Mediated in Apple |
|
| CAO Qiu-fen,YUE Xin-li,MENG Yu-ping,JI Lan,FAN Xin-ping.Effects of Ultrasonication Treatment on Genetic Transformation by Pollen-Mediated in Apple[J].Acta Agriculturae Boreali-Sinica,2005,20(2):16-18. | |
| Authors: | CAO Qiu-fen YUE Xin-li MENG Yu-ping JI Lan FAN Xin-ping |
| Institution: | CAO Qiu-fen~1,YUE Xin-li~2,MENG Yu-ping~1,JI Lan~2,FAN Xin-ping~3 |
| Abstract: | The article discussed the effects of additional ultrasonication treatment on the germination of apple(Malus pumila Mill) pollen during pollen-mediated genetic transformation of apple cultivars Danxia and Gala. In order to find out suitable buffer solution and ultrasonication parameters, different pH values, cane sugar concentrations of buffer and various powers, treatment times, treatment number of ultrasonication were set up and examined through experiment. The results showed that the suitable buffer pH value fit for apple pollen treatment was 5.4 and the suitable concentration of cane sugar 10%. It was also indicated that such parameters as the power, time and treatment number of ultrasonication had significant effect on the broken rate and the germinated rate of apple pollen. |
| Keywords: | Apple Pollen Genetic transformation Ultrasonication |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |