| 木豆高频不定芽再生及体细胞遗传变异的分子检测 |
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| 引用本文： | 赵庆鹤,崔博文,乔光,文晓鹏.木豆高频不定芽再生及体细胞遗传变异的分子检测[J].种子,2010,29(7). |
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| 作者姓名： | 赵庆鹤 崔博文 乔光 文晓鹏 |
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| 作者单位： | 1. 贵州大学/贵州省农业生物工程重点实验室,贵阳,550025;贵州大学林学院,贵阳,550025
2. 贵州大学/贵州省农业生物工程重点实验室,贵阳,550025 |
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| 摘 要： | 以木豆子叶节为外植体,建立高频不定芽再生体系,并对继代丛芽的遗传变异进行分子检测。结果表明:子叶节不定芽再生的最佳培养基为EC6附加4.0mg/LBA和0.1mg/LIBA;丛芽增殖培养以EC6附加4.5mg/LBA、0.1mg/LIBA和0.4mg/LKT效果最佳。同时,随机挑选继代第5代的20个丛芽提取基因组DNA,采用45个ISSR引物对基因组DNA进行分子检测,结果显示,在所用引物范围内均未检测到变异。
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| 关 键 词： | 木豆 再生体系 器官发生型 体细胞变异 分子标记 |
Efficient Regeneration from Cotyledonary Nodes of Pigeon Pea Via Organogenesis and Genetic Fidelity of Adventitious Shoots as Revealed by ISSR Markers |
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| ZHAO Qing-he,CUI Bo-wen,QIAO Guang,WEN Xiao-peng.Efficient Regeneration from Cotyledonary Nodes of Pigeon Pea Via Organogenesis and Genetic Fidelity of Adventitious Shoots as Revealed by ISSR Markers[J].Seed,2010,29(7). |
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| Authors: | ZHAO Qing-he CUI Bo-wen QIAO Guang WEN Xiao-peng |
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| Institution: | ZHAO Qing-he1,2,CUI Bo-wen1,QIAO Guang1,WEN Xiao-peng1(1.Guizhou Key Laboratory of Agricultural Bioengineering,Guizhou University,Guiyang 550025,China,2.College of Forestry,China) |
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| Abstract: | With cotyledonary nodes of pigeon pea(Cajanus cajan) as explant to establish efficient regeneration system and detect genetic variation of adventious buds of Pigeon pea(Cajanus cajan) with molecule biology method.The result showed that the EC6 supplemented with both 4.0 mg /L BA and 0.1 mg /L IBA was the best medium for cotyledonary nodes regeneration efficiently.Bud regeneration cultivation was the best in EC6 medium supplement with the combination of 4.5 mg /L BA,0.1 mg /L IBA and 0.4 mg /L KT.To investig... |
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| Keywords: | pigeon pea(Cajanus cajan) regeneration system organogenesis somaclonal variation molecular marker |
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