| 新冬26小麦幼胚盾片再生体系的建立 |
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| 引用本文： | 王勇,任崴,祝长青.新冬26小麦幼胚盾片再生体系的建立[J].种子,2011,30(8). |
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| 作者姓名： | 王勇 任崴 祝长青 |
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| 作者单位： | 1. 新疆师范大学分子生物学与生物信息研究室,乌鲁木齐,830054
2. 中国科学院新疆生态与地理研究所,乌鲁木齐,830011 |
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| 基金项目： | 国家自然科学基金(30960044); 自治区科技厅高技术项目(201011110) |
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| 摘 要： | 以新冬26小麦的幼胚盾片为实验材料,研究不同激素及其不同浓度对愈伤组织诱导和分化的影响.结果表明:2,4-D和Dicamba组合诱导的愈伤组织分化率显著优于单独添加2,4-D或Dicamba.分化培养基中添加低浓度的ZT有利于根的分化.诱导培养基采用MS附加2,4-D 1.5 mg/L和Dicamba 0.5 mg/L,分化培养基采用R附加ZT1 mg/L和2,4-D0.01 mg/L对愈伤组织分化效果为最佳,绿苗分化率最高可达90％.本研究结果为建立高效、稳定的新疆主栽小麦品种的遗传转化系统奠定了基础.
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| 关 键 词： | 新冬26 幼胚盾片 再生 |
Establishment of Wheat Plant Regeneration System for Immature Embryo Scutella of Xindong No.26 |
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| WANG Yong,REN Wei,ZHU Chang-qing.Establishment of Wheat Plant Regeneration System for Immature Embryo Scutella of Xindong No.26[J].Seed,2011,30(8). |
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| Authors: | WANG Yong REN Wei ZHU Chang-qing |
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| Institution: | WANG Yong1,REN Wei2,ZHU Chang-qing1(1.Research Room of Molecular Biology and Informatics,Xinjiang Normal University,Urumqi 830054,China,2.Xinjiang Institute of Ecology and Geography Research,Chinese Academy of Sciences,Urumqi 830011,China) |
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| Abstract: | Immature embryo scutella of Xindong No.26 were used as explants to study the effect of different combinations of auxins and concentrations on callus induction and differentiation of callus.The result showed that differentiation rate of callus induced by 2,4-D with Dicamba in medium was significantly better than that induced by 2,4-D or Dicamba alone.Differentiation medium containing lower concentration of ZT was propitious to differentiation of root.MS medium containing 2,4-D1.5mg/L and Dicamba 0.5mg/L for ... |
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| Keywords: | Xindong No 26 immture embryo scutellum regeneration |
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