| 转基因甜菜H7-1实时荧光PCR检测方法 |
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| 引用本文： | 张舒亚,吕蓉,高琴,刘月明,李想,郭云霞,李富威.转基因甜菜H7-1实时荧光PCR检测方法[J].种子,2012,31(1):53-56. |
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| 作者姓名： | 张舒亚 吕蓉 高琴 刘月明 李想 郭云霞 李富威 |
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| 作者单位： | 1. 上海出入境检验检疫局,上海,200135
2. 上海海洋大学,上海,201306 |
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| 基金项目： | 上海市技术性贸易措施应对专项(10TBT009);出入境检验检疫行业标准制定项目(2010B205) |
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| 摘 要： | 运用实时荧光PCR方法对转基因甜菜H7-1外源基因FMV 35 S启动子、E93’终止子、CP 4-EPSPS外源基因和品系特异性进行了检测.建立了快速、准确的转基因甜菜H7-1转基因成分的PCR检测方法.本研究采用的FMV 35 S启动子、E93’终止子、CP 4-EPSPS和品系特异性检测引物探针能有效检测转基因甜菜H7-1中转基因成分,具有特异性,且灵敏度达到0.01％.
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| 关 键 词： | 转基因甜菜H 7-1 实时荧光PCR 检测 |
Detection of Genetically Modified Sugar Beet H 7-1 Using Real-time PCR |
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| ZHANG Shu-ya
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L Rong
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GAO Qin
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LIU Yue-ming
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LI Xiang
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GUO Yun-xia
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LI Fu-wei.Detection of Genetically Modified Sugar Beet H 7-1 Using Real-time PCR[J].Seed,2012,31(1):53-56. |
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| Authors: | ZHANG Shu-ya L Rong GAO Qin LIU Yue-ming LI Xiang GUO Yun-xia LI Fu-wei |
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| Institution: | 1.Shanghai Entry-Exit Inspection and Quarantine,Shanghai 200135,China; 2.Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | The paper designed to detect exogenous transgenic FMV 35 S promoter,E 9 3’terminator,target CP4-EPSPS gene and event-specific nucleotide sequence from genetically modified sugar beet H 7-1 by real-time PCR.The accurate and fast PCR detection methods and system were established in genetically modified sugar beet H 7-1. |
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| Keywords: | genetically modified sugar beet H 7-1 real-time PCR detection |
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