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| 适合RAPD分析的三种玉米基因组DNA提取方法比较 | |
| 引用本文： | 朱英,陶刚,刘作易.适合RAPD分析的三种玉米基因组DNA提取方法比较[J].种子,2006,25(1):16-18. |
| 作者姓名： | 朱英 陶刚 刘作易 |
| 作者单位： | 1. 贵州省农业生物技术重点实验室,贵阳,550006;贵州省农业科学院生物技术研究所,贵阳,550006 2. 贵州省农业生物技术重点实验室,贵阳,550006;贵州省农业科学院植物保护研究所,贵阳,550006 3. 贵州省农业生物技术重点实验室,贵阳,550006;贵州省农业科学院,贵阳,550006 |
| 基金项目： | 贵州省科技攻关项目[黔科合农社字（2002）1136号];贵州省农科院重点项目. |
| 摘 要： | 本文通过对3种玉米基因组DNA提取方法的比较得出，DNA提取时采用一次氯仿／异戊醇抽提方法，得到的DNA质和量都较为理想，并且能够达到RAPD实验的要求。 |
| 关 键 词： | 玉米 基因组DNA DNA提取 RAPD分析 |
| 收稿时间： | 2005-09-25 |
| 修稿时间： | 2005-09-25 |
A Comparison of Three Maize Genomic DNA Extraction Methods for RAPD Analysis |
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| Zhu Ying,Tao Gang,Liu Zuoyi.A Comparison of Three Maize Genomic DNA Extraction Methods for RAPD Analysis[J].Seed,2006,25(1):16-18. | |
| Authors: | Zhu Ying Tao Gang Liu Zuoyi |
| Institution: | 1. The Key Laboratory for Agri. Biotechnology of Guizhou, Guiyang,550006; 2. Institute of Biotechnology, Guizhou Academy of Agri. Sci, Guiyang 550006 ; 3. Institute of Plant Protection, Guizhou Academy of Agri. Sci, Guiyang 550006 ; 4. Guizhou Academy of Agri. Sci, Guiyang 550006 |
| Abstract: | In this paper,three genomic DNA extraction methods of maize were conducted to show that the best one that can not only satisfy the requirements of radom amplified polymorphic DNA(RAPD) analysis but also be the simplest to operate with rapid and economical effects.The purified and heavier DNA molecule could be obtained by one time of extraction with the organic solvent such as phenol-chloroform,and was tested to be desired for the further experiments by agarose gel electrophoresis and RAPD. |
| Keywords: | Maize Genomic DNA DNA extraction Random amplified polymorphic DNA(RAPD) analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |