| 发酵木糖高产乙醇酵母菌株的选育 |
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| 引用本文： | 解蕙铭,闵伟红,张丹.发酵木糖高产乙醇酵母菌株的选育[J].农产品加工．学刊,2011(5):32-35. |
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| 作者姓名： | 解蕙铭 闵伟红 张丹 |
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| 作者单位： | 1. 吉林农业大学食品科学与工程学院,吉林,长春,130118;长春医学高等专科学校生物工程系,吉林,长春,130031
2. 吉林农业大学食品科学与工程学院,吉林,长春,130118 |
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| 摘 要： | 筛选获得的季也蒙毕赤酵母(Pichia guilliermondii)为出发菌株,采用紫外线和亚硝基胍(NTG)复合诱变,筛选高产乙醇突变株。结果表明,紫外诱变菌最佳稀释度为1×10-5,最佳照射时间为25 s;NTG诱变菌最佳稀释度为1×10-6,最佳诱变时间为40 min,经3轮紫外和亚硝基胍(NTG)交替诱变,获得高产乙醇突变株C3-10,其乙醇产量最高为13.2%,比原菌乙醇产量增加了8.05%,5次传代表现出较好的遗传稳定性。
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| 关 键 词： | 乙醇 木糖 诱变育种 |
Mutation Breeding of Fermentating Xylose High-yield Ethanol Yeast Strains |
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| Xie Huiming,Min Weihong,Zhang dan.Mutation Breeding of Fermentating Xylose High-yield Ethanol Yeast Strains[J].Nongchanpin Jlagong.Xuekan,2011(5):32-35. |
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| Authors: | Xie Huiming Min Weihong Zhang dan |
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| Institution: | Xie Huiming1,2,Min Weihong1,Zhang dan1(1.Food Engineer College,Jilin Agricultural University,Changchun,Jilin 130118,China,2.Bioengineering Department,Changchun Medical College,Jilin 130031,China) |
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| Abstract: | This paper takes UV and N-methyl-N'-nitro-nitrosoguanidine(NTG) as mutagenesis agents,using Screening obtaining pichia guilliermondii as starting strain,in order to obtain the high-yield strain of ethanol.The results show that the best bacterium diluted concentration used for UV mutagenesis is 1×10-5,the best time of UV exposure time is 25 s;the best bacterium diluted concentration used for NTG mutagenesis is 1×10-6,the best time of NTG mutagenesis is 40 min,after Three-circled alternation of UV and NTG mutagenesis for strain screening,we obtain high-yield strain of ethanol,among the total mutant strains,the mutant strain C3-10 generated the tallest ethanol quantity,is 13.2%,comparing with prototroph strain generated ethanol after fermentation has increased to 8.05%,it shows that go down to 5 posterity has good genetic stability. |
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| Keywords: | ethanol xylose mutation breeding |
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