| HPLC同时测定花生壳中绿原酸3,4—二咖啡酰奎尼酸和木犀草素 |
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| 引用本文： | 王晴晴,张盛,李银花,刘仲华.HPLC同时测定花生壳中绿原酸3,4—二咖啡酰奎尼酸和木犀草素[J].农产品加工．学刊,2011(6):69-70,78. |
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| 作者姓名： | 王晴晴 张盛 李银花 刘仲华 |
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| 作者单位： | 1. 国家植物功能成分利用工程技术研究中心,湖南长沙,410128
2. 国家植物功能成分利用工程技术研究中心,湖南长沙,410128;湖南农业大学,植物资源工程系,湖南长沙,410128 |
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| 基金项目： | 特色植物资源高值化利用关键技术研究与开发(7FJ1005) |
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| 摘 要： | 建立了同时测定花生壳中绿原酸、3,4-二咖啡酰奎尼酸和木犀草素3种有效成分含量的反相高效液相色谱方法。采用Kromasil C18柱(200 mm×4.6 mm,5μm),乙腈和0.1%甲酸水溶液梯度洗脱,流速为1.0 mL/min,检测波长340 nm,柱温27℃。在该色谱条件下,绿原酸、3,4-二咖啡酰奎尼酸和木犀草素的质量浓度分别为3.75~60μg/mL(R2=0.999 9),3.63~58.08μg/mL(R2=0.999 9),17.25~276μg/mL(R2=0.999 7)内与色谱峰面积呈现良好的线性关系,平均加标回收率(n=5)为121.6%,97.79%,124.35%,RSD分别为0.71%,1.49%,0.44%。该分析方法快速准确、重现性好,可应用于花生壳资源产业开发中质量标准的建立和完善。
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| 关 键 词： | 花生壳 绿原酸 3 4-二咖啡酰奎尼酸 木犀草素 |
Simultaneous Determination of Chlorogenic Acid,3,4-O-dicaffeoylquinic Acid and Luteolin in Peanut Hull by HPLC |
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| Wang Qingqing,Zhang Sheng,Li Yinhua,Liu Zhonghua.Simultaneous Determination of Chlorogenic Acid,3,4-O-dicaffeoylquinic Acid and Luteolin in Peanut Hull by HPLC[J].Nongchanpin Jlagong.Xuekan,2011(6):69-70,78. |
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| Authors: | Wang Qingqing Zhang Sheng Li Yinhua Liu Zhonghua |
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| Institution: | Wang Qingqing1,Zhang Sheng1,2,Li Yinhua1,Liu Zhonghua1,2(1.National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanical,Changsha,Hu'nan 410128,China,2.Department of Botanical Resources Engineering,Hu'nan Agricultural University,China) |
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| Abstract: | A method of reversed-phase high performance liquid chromatography is established for the simultaneous determination of chlorogenic acid,3,4-O-dicaffeoylquinic acid and luteolin in peanut hull.Separation is achieved using a Kromasil C18 column(4.6 mm × 200 mm,5 μm),under the gradient elution with the mobile phase of acetonitrile and 0.1% formic acid / water,flow rate is 1.0 mL/min,detected at 340 nm and under 27 ℃.The linear ranges of 3 components are 3.75~ 60 μg/mL(R2=0.999 9),3.63 ~58.08 μg/mL(R2=0.999 9) and 17.25 ~ 276 μg/mL(R2=0.999 7) respectively.The average recoverises(n=5) are 121.60%,97.79%,124.35% and with RSD of 0.71%,1.49%,0.44% respectively.The method is simple,rapid and highly sensitive,suitable for the quality control of peanut hull. |
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| Keywords: | peanut hull chlorogenic acid 3 4-O-dicaffoylquinic acid luteolin |
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