| 苯酚—硫酸法测定广东虫草菌丝体多糖含量的研究 |
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| 引用本文： | 郑必胜,唐芳勇,闫文娟,李泰辉.苯酚—硫酸法测定广东虫草菌丝体多糖含量的研究[J].农产品加工．学刊,2008(8). |
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| 作者姓名： | 郑必胜 唐芳勇 闫文娟 李泰辉 |
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| 作者单位： | 1. 华南理工大学,轻工与食品学院,广东,广州,510640
2. 广东省微生物研究所,广东省菌株保藏与应用重点实验室,广东,广州,510070 |
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| 基金项目： | 广东省粤港招标东莞专项,2008-2010(2007168604) |
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| 摘 要： | 以精制后的广东虫草菌丝体多糖为研究对象,通过单因素实验,对苯酚—硫酸法测定广东虫草菌丝体精制多糖含量的显色条件进行优选,并对该法的精密度、稳定性、加样回收率,以及多糖液中色素、蛋白质的干扰等进行了研究。结果表明,该测定方法简便灵敏,准确性高,稳定性好,测定结果可靠,且不受色素及蛋白质的影响。用苯酚—硫酸法在最佳显色条件下测得的广东虫草菌丝体多糖含量为10.52%。
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| 关 键 词： | 广东虫草 苯酚—硫酸法 多糖 |
Study on Polysaccharides from Guangdong Cordyceps Mycelium by Phenol-sulfuric Acid Method |
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| Zheng Bisheng,Tang Fangyong,Yan Wenjuan,Li Taihui.Study on Polysaccharides from Guangdong Cordyceps Mycelium by Phenol-sulfuric Acid Method[J].Nongchanpin Jlagong.Xuekan,2008(8). |
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| Authors: | Zheng Bisheng Tang Fangyong Yan Wenjuan Li Taihui |
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| Abstract: | Polysaccharides from Guangdong cordyceps mycelium. In the paper,they were refined and then selected as a research object to perform single factor experiment,optimizing the chromogenic condition of phenol-sulfuric acid method with regard to the refined polysaccharides. Some parameter associated with this method,such as precision,stability,recovery rate as well as the interference by pigment and protein in the polysaccharides samples,were also studied in this paper. The results show that,the assay method whic... |
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| Keywords: | Guangdong cordyceps phenol-sulfuric acid method polysaccharides |
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