| 酶法合成葡萄糖基-L-抗坏血酸的产酶条件及转化条件优化 |
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| 引用本文： | 曹新志,刘芳,明红梅,武玉娟.酶法合成葡萄糖基-L-抗坏血酸的产酶条件及转化条件优化[J].农产品加工．学刊,2008(1):19-22,28. |
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| 作者姓名： | 曹新志 刘芳 明红梅 武玉娟 |
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| 作者单位： | 四川理工学院,生物工程系,四川,自贡,643000 |
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| 基金项目： | 四川省自贡市科学技术研究项目 |
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| 摘 要： | 采用单因素实验确立微生物发酵法产环糊精糖基转移酶培养基的基本条件和工艺条件,利用此酶催化L-抗坏血酸和糖基供体β-环糊精合成葡萄糖基-L-抗坏血酸(AA-2G),并通过正交实验考察不同加酶量、反应温度、pH值等因素,确立环糊精糖基转移酶合成产物的最佳条件为:浓度为0.04mol/L的L-抗坏血酸,酶量20mL,pH值7.0,温度50℃下反应15h。
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| 关 键 词： | 葡萄糖基-L-抗坏血酸 环糊精糖基转移酶 β-环糊精 |
| 文章编号： | 1671-9646（2008）01-0019-04 |
| 收稿时间： | 2007-12-25 |
| 修稿时间： | 2007年12月25 |
The Optimization of the Enzyme Producing Condition and the Transformation Condition to Prepare Glucopyranosyl-L-ascorbid Acid |
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| Cao Xinzhi,Liu Fang,Ming Hongmei,Wu Yujuan.The Optimization of the Enzyme Producing Condition and the Transformation Condition to Prepare Glucopyranosyl-L-ascorbid Acid[J].Nongchanpin Jlagong.Xuekan,2008(1):19-22,28. |
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| Authors: | Cao Xinzhi Liu Fang Ming Hongmei Wu Yujuan |
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| Institution: | Cao Xinzhi,Liu Fang,Ming Hongmei,Wu Yujuan (College of Biology Engineering,Sichuang University of Science & Engineering,Zigong,Sichuang 643000,China) |
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| Abstract: | The culture medium component and culture condition for producing cyclodextrin clycosyltransferase by Bacillus alkalophilus7-12 was primarily established by single sactor design.The enzyme was used to transform L-ascorbic acid to glucopyranosyl-L-ascorbid acid(AA-2G).Its optimal transformation condition was established by orthogonal test design.The best condition is 0.04 mol/L VC,the enzyme volume 20 mL,pH7.0,reacting under 50 ℃ for 15 h. |
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| Keywords: | glucopyranosyl-L-ascorbid acid cyclodextrin clycosyltransferase β-cyclodextrin |
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