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超声波提取菜籽粕中水溶性蛋白工艺的研究
引用本文:吴文锦,杜欣,汪兰,于巍,乔宇,廖李,程薇,熊光权.超声波提取菜籽粕中水溶性蛋白工艺的研究[J].农产品加工.学刊,2012(2):15-19.
作者姓名:吴文锦  杜欣  汪兰  于巍  乔宇  廖李  程薇  熊光权
作者单位:湖北省农业科学院农产品加工与核农技术研究所湖北省农业科技创新中心农产品加工分中心
基金项目:农业部公益性科研专项资助(200903043)
摘    要:以醇变性菜籽粕为原料,采用超声波提取水溶性蛋白,在单因素试验基础上,采用二次正交旋转组合方法,优化得到最佳提取条件为:pH值13,提取时间12 min,提取温度35℃,醇变性菜籽粕质量分数3%,在此条件下,水溶性蛋白质量分数达45.97%。

关 键 词:菜籽粕  超声  二次回归正交旋转组合

Extraction of Water-soluble Protein of Rapeseed by Ultrasonic
WU Wen-jin,DU Xin,WANG Lan,YU Wei,QIAO Yu,LIAO li,CHENG Wei,XIONG Guang-quan.Extraction of Water-soluble Protein of Rapeseed by Ultrasonic[J].Nongchanpin Jlagong.Xuekan,2012(2):15-19.
Authors:WU Wen-jin  DU Xin  WANG Lan  YU Wei  QIAO Yu  LIAO li  CHENG Wei  XIONG Guang-quan
Institution:(Institute of Processing of Agricultural Produce and Nuclear Agricultural Research,Hubei Academy of Agricultural Science,Wuhan,Hubei 430064,China)
Abstract:In this experiment,the water-soluble protein is obtained with alcohol leached rapeseed as material by ultrasonic. Based on the single factor tests,the extraction parameters are optimized by quadratic regression orthogonal rotary method.The results show that the optimum conditions are pH value 13,extraction time 12 min,extraction temperature 35 ℃,alcohol leached rapeseed content 3%.Under these conditions,the content of water-soluble protein can reach to 45.97%.
Keywords:rapeseed  ultrasonic  quadratic regression orthogonal rotary method
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