| 鸭血中SOD的提取及稳定性研究 |
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| 引用本文： | 周亚迪,李诚,康斌,范成敏,刘亚. 鸭血中SOD的提取及稳定性研究[J]. 农产品加工．学刊, 2011, 0(10): 21-24 |
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| 作者姓名： | 周亚迪 李诚 康斌 范成敏 刘亚 |
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| 作者单位： | 四川农业大学食品学院 |
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| 基金项目： | 2010年教育部国家级创新性实验(101062625) |
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| 摘 要： | 为充分利用鸭血资源,以新鲜鸭血为原料,利用响应面分析法对鸭血中超氧化物歧化酶(SOD)的提取工艺进行优化,并初步研究了SOD在不同条件下的稳定性。结果表明,乙醇与氯仿的体积比4∶5,溶血球与沉淀剂的体积比1∶1,静置时间25.5 min,SOD比活为1 000.91 U/mg;在pH值4.0～6.0,50℃温度以下SOD具有良好的稳定性。
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| 关 键 词： | 鸭血 超氧化物歧化酶 响应面优化 稳定性 |
Extraction and Stability of Superoxide Dismutase from Duck Blood |
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| Zhou Yadi,Li Cheng,Kang Bin,Fan Chengmin,Liu Ya. Extraction and Stability of Superoxide Dismutase from Duck Blood[J]. Nongchanpin Jlagong.Xuekan, 2011, 0(10): 21-24 |
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| Authors: | Zhou Yadi Li Cheng Kang Bin Fan Chengmin Liu Ya |
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| Affiliation: | (Food Science College,Sichuan Agricultural University,Ya’an,Sichuan 625014,China) |
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| Abstract: | In order to develop the duck blood,using fresh duck blood as material,the denatureation processes of duck blood of SOD are optimized by response surface methodology.Under different conditions of SOD in stability are studied.The results show that the volume ratio of ethanol and chloroform 4∶5,Hemolytic ball and precipitant volume ratio of 1∶1,holding time 25.5 min,SOD 1 000.91 U/ mg.SOD at pH 4.0 ~ 6.0 has a good stability,temperature in 50 degrees here are good heat resistance. |
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| Keywords: | duck blood SOD response surface optimization stability |
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