| 聚赖氨酸微囊固定化胰蛋白酶的研究 |
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| 引用本文： | 曹泽虹,苗敬芝,高明侠,王文治,秦卫东,吕兆启.聚赖氨酸微囊固定化胰蛋白酶的研究[J].农产品加工．学刊,2006(8):39-41. |
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| 作者姓名： | 曹泽虹 苗敬芝 高明侠 王文治 秦卫东 吕兆启 |
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| 作者单位： | 徐州工程学院,食品工程系,江苏,徐州,221008 |
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| 摘 要： | 以海藻酸钠为载体,氯化钙为聚凝剂,聚赖氨酸为微囊膜固定化胰蛋白酶。结果表明,以 4%的海藻酸钙为载体,2%的氯化钙为凝聚剂和 0.06%的聚赖氨酸为微囊膜,加酶量为 6%,温度为 70 ℃,pH 值为 7.5,Km 值为4.51 mg/mL,在 4 ℃条件下固定化胰蛋白酶的半衰期为 35 d。
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| 关 键 词： | 聚赖氨酸 微囊 固定化 固定化胰蛋白酶 |
| 文章编号： | 1671-9646（2006）08-0039-03 |
| 收稿时间： | 2006-08-01 |
| 修稿时间： | 2006年8月1日 |
Study on Immobilization of Trypsin by Polylysine Microenpsulation |
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| Cao Zehong,Miao Jingzhi,Gao Mingxia,Wang WenZhi,Qin Weidong,Lu Zhaoqi.Study on Immobilization of Trypsin by Polylysine Microenpsulation[J].Nongchanpin Jlagong.Xuekan,2006(8):39-41. |
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| Authors: | Cao Zehong Miao Jingzhi Gao Mingxia Wang WenZhi Qin Weidong Lu Zhaoqi |
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| Abstract: | In this paper,trypsinase was immobilized on alginic acid vector by solidify of CaCl2 and microencapsulated with polylysine. The results showed that the high rate of recovery of immobilized enzyme could be obtained under the conditions of 4% alginic acid,2% CaCl2,5% trypsin,0.06% polylysine,70 ℃ and pH 7.5. The extent of the optimum temperature of immobilized trypsinase was large than non-immobilized trypsin. The optimum pH and apparent Km value of the immobilized enzyme decreaed slightly,the half-life of immobilized trypsinase was 35 d. |
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| Keywords: | polylysine microencapsulation immobilization immobilization trypsinase |
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