双酶法水解罗非鱼下脚料制备降血糖肽的工艺研究

以水解度和α-葡萄糖苷酶抑制率为评价指标,确定双酶复合水解罗非鱼下脚料的方案,并通过单因素试验和正交试验进行优化,最后得到最适酶解工艺参数为先在碱性蛋白酶在温度50℃,加酶量10000 U/g,pH值9.5,底物质量分数6%条件下水解;再在胰蛋白酶在温度37℃,加酶量10000 U/g,pH值8条件下水解100 min。此工艺条件下罗非鱼下脚料水解度、水解产物的α-葡萄糖苷酶抑制率分别为48.26%和41.46%。

Study on Preparation of Hypoglycemic Peptide from Tilapia Leftovers by Double Enzyme Hydrolysis

Taking the degree of hydrolysis and the inhibition rate of alpha glucosidase as the evaluation index,the scheme of compound hydrolysis of tilapia leftovers by double enzymes was determined.Through mono-factor and orthogonal experiments,the optimum technological parameters of enzymatic hydrolysis were as follows:Firstly,alkaline protease was used to hydrolyze at the condition of temperature 50℃,enzyme dosage 10000 U/g,pH 9.5 and substrate concentration 6%;then trypsin was used to hydrolyze at the condition of temperature 37℃,enzyme amount 10000 U/g and pH 8 for 100 min.Under the conditions,the hydrolysis degree and the inhibition rate of alpha glucosidase were 48.26%and 41.46%,respectively.