| 双酶法水解黑豆豆渣制备可溶性蛋白 |
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| 引用本文： | 任海伟,王常青,苏槟楠.双酶法水解黑豆豆渣制备可溶性蛋白[J].农产品加工．学刊,2007(5):39-41. |
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| 作者姓名： | 任海伟 王常青 苏槟楠 |
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| 作者单位： | 1. 山西大学,生命科学与技术学院,山西,太原,030006
2. 山西省生物研究所,山西,太原,030006 |
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| 摘 要： | 研究了不同蛋白酶水解黑豆豆渣,制备可溶性蛋白的工艺。结果表明,先用木瓜蛋白酶,在温度50℃,pH值为6.5的条件下作用3h,再用碱性蛋白酶,在pH值为9.0的条件下作用3h,黑豆豆渣的蛋白溶出率可以达到68.43%,氨基酸态氮含量控制在2.17%,溶解度为98.25%。
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| 关 键 词： | 黑豆豆渣 蛋白酶 蛋白溶出率 |
| 文章编号： | 1671-9646（2007）05-0039-03 |
| 修稿时间： | 2007-04-02 |
Preparation of Soluble Protein from Black-soybean Dregs by Bienzyme Hydrolysis |
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| Ren Haiwei,Wang Changqing,Su Binnan.Preparation of Soluble Protein from Black-soybean Dregs by Bienzyme Hydrolysis[J].Nongchanpin Jlagong.Xuekan,2007(5):39-41. |
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| Authors: | Ren Haiwei Wang Changqing Su Binnan |
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| Institution: | 1. Institute of Life Science and Technology, Shanxi University, Taiyuan, Shanxi 030006, China; 2. Shanxi Institute of Biology, Taiyuan, Shanxi 030006, China |
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| Abstract: | The technological process of different proteinases hydrolysis preparation of soluble protein from black-soybean dregs was studied.When the black-soybean dregs were hydrolyzed by papain in the condition of pH6.5?50 ℃ for 3 hours,and then,hydrolyzed continuously by protease proteinases in the condition of pH9.0 for 3 hours,the extraction rate of soluble protein was up to 68.43%.The amount of ammonia nitrogen was 2.17% and the degree of solubility was 98.25% in this condition. |
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| Keywords: | black-soybean dregs proteinases extraction rate of soluble protein |
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