| 优良玉米自交系丹598和丹988幼胚愈伤组织的诱导和再生 |
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| 引用本文： | 牛亚玲,王丕武,曲静,高雯雯,王俊玲,王薇,兰磊.优良玉米自交系丹598和丹988幼胚愈伤组织的诱导和再生[J].分子植物育种,2009,7(4):716-720. |
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| 作者姓名： | 牛亚玲 王丕武 曲静 高雯雯 王俊玲 王薇 兰磊 |
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| 作者单位： | 1. 吉林农业大学农学院,长春,130118
2. 吉林农业大学生物技术学院,长春,130118 |
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| 基金项目： | 国家转基因专项，吉林省重大科技公关项目 |
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| 摘 要： | 采用玉米自交系丹598和丹988的幼胚作为外植体,以N6和MB培养基为基本培养基,探讨基因型、培养基种类、激素种类和浓度对愈伤组织诱导率的影响,分析不同基因型愈伤组织生长旺盛时期的异同.结果显示:基因型对愈伤组织诱导率高低影响显著,且基因型和培养基之间存在相互作用.2,4-D对玉米幼胚愈伤组织的诱导形成是必需的,浓度为2.0mg/L时大多能诱导出胚性愈伤组织.本试验建立了玉米自交系丹598和丹988的优良再生体系,为以后的基因转化工作打下了良好基础.
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| 关 键 词： | 玉米 幼胚 愈伤组织 诱导 再生体系 |
Embryogenic Calli Induction and Plant Regeneration in Excellent Maize Inbred Lines Dan598 and Dan988 |
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| NiuYaling,WangPiwu,Qujing,GaoWenwen,WangJunling,Wangwei,Lanlei.Embryogenic Calli Induction and Plant Regeneration in Excellent Maize Inbred Lines Dan598 and Dan988[J].Molecular Plant Breeding,2009,7(4):716-720. |
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| Authors: | NiuYaling WangPiwu Qujing GaoWenwen WangJunling Wangwei Lanlei |
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| Institution: | Niu Yaling 1 Wang Piwu 1 Qu jing 2 Gao Wenwen 2 Wang Junling 2 Wang wei 2 Lan lei 2 1 College of Agronomy,Jilin Agricultural University,Changchun,130118,2 College of Biotechnology |
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| Abstract: | Callus was induced from immature embryos of maize inbred lines Dan598 and Dan988 on MB medium and N6 medium. The effects of different genotypes, different mediums and different concentrations of phytohormone on the callus induction of immature embryos were studied. The effects of genotypes on different callus during the vigorously growing stage were also analyzed. The results indicated that genotypes have significant effects on callus induction. Both genotype and medium affected the frequency of embryogenic... |
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| Keywords: | Maize Immature embryos Callus Induction Regeneration system |
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