| 茄子SSR标记在番茄上的通用性分析 |
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| 引用本文： | 邵光金,牛玉,乐素菊,汪文毅,汪国平.茄子SSR标记在番茄上的通用性分析[J].分子植物育种,2010,8(5). |
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| 作者姓名： | 邵光金 牛玉 乐素菊 汪文毅 汪国平 |
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| 作者单位： | 1. 华南农业大学园艺学院,广州,510642
2. 仲恺农业工程学院生命科学学院,广州,510225 |
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| 基金项目： | 国家自然科学基金，广东省科技厅国际合作项目，科技部国际合作项目 |
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| 摘 要： | 微卫星标记(SSR)是分子育种中常用的遗传标记之一,但目前报道的番茄SSR标记并不能满足番茄分子育种的需要,有必要发展出更多的SSR标记,近缘物种转移法是一种发展SSR标记简便快速的方法。本研究分析了茄子SSR标记在番茄上的通用性情况,结果表明:300对茄子SSR引物中有111对能在番茄基因组DNA上扩增出产物,97对引物扩增出的带型在茄子、番茄间相似程度高,标记通用率为32.3%;EST-SSR比基因组SSR的通用性更好,前者通用率为36.6%,后者为30.0%。将这些标记转移到番茄上,能节省开发番茄SSR标记的成本。
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| 关 键 词： | 茄子 番茄 微卫星标记 通用性 |
Transferability of Eggplant SSRs to Tomato |
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| Shao Guangjin,Niu Yu,Yue Suju,Wang Wenyi,Wang Guoping.Transferability of Eggplant SSRs to Tomato[J].Molecular Plant Breeding,2010,8(5). |
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| Authors: | Shao Guangjin Niu Yu Yue Suju Wang Wenyi Wang Guoping |
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| Institution: | Shao Guangjin1 NiuYu1 Yue Suju2 Wang Wenyi 1 Wang Guoping 1 1 College of Horticulture,South China Agricultural University,Guangzhou,510642,2 College of Life Sciences,Zhongkai University of Agriculture and Engineering,510225 |
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| Abstract: | Simple sequence repeats(SSR) is one of the marker systems widely used in molecular breeding.However,only a limited numberof SSRs have been reported in tomato by now and cannot satisfy the demand of molecularbreeding,it is still necessary to develop more SSRs in tomato.SSR transfer between related species is a feasible method to develop new SSR markers.The current study has tested the transferability of eggplant SSRs to tomato.A total of 300 eggplant SSRs were tested,111 of which could successfully amplified... |
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| Keywords: | Eggplant Tomato Simple sequence repeats(SSR) Transferability |
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