| 蓖麻GST的基因克隆及生物信息学分析 |
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| 引用本文： | 温琦,梁塔娜,张艳欣,李丽丽,张幽静,黄凤兰.蓖麻GST的基因克隆及生物信息学分析[J].分子植物育种,2020(7):2161-2166. |
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| 作者姓名： | 温琦 梁塔娜 张艳欣 李丽丽 张幽静 黄凤兰 |
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| 作者单位： | 内蒙古民族大学生命科学与食品学院;内蒙古民族大学;内蒙古牙克石市乌尔其汉镇畜牧兽医草原站 |
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| 基金项目： | 国家自然科学基金项目(31860071);内蒙古自治区自然科学基金面上项目(2017MS0339);内蒙古自治区草原英才创新团队——蓖麻分子育种创新研究团队支持项目(2017);内蒙古民族大学自治区科技储备项目子课题(2018NDCB05-2);内蒙古自治区高校蓖麻产业工程技术研究中心开放基金项目(MDK2018014)共同资助。 |
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| 摘 要： | 谷胱甘肽S-转移酶(glutathione S-transferase,GST)是谷胱甘肽结合反应的关键酶,催化谷胱甘肽结合反应的起始步骤,主要存在于胞液中,有多种形式,其在病理学上有一定的重要性。本研究通过克隆GST基因以及对其进行相关的生物信息学分析。利用聚合酶链式反应(polymerase chain reaction,PCR)技术扩增该基因,经分析该基因可编码219个氨基酸,并且通过相关分析得到这219个氨基酸组成的蛋白为稳定的亲水性蛋白,有一个明显的区段存在信号肽,在该蛋白中α-螺旋和无规则卷曲是主要的二级结构方式,根据序列同源比对,蓖麻、麻疯树和榴莲聚类到一起,说明相对于其他物种,它们的同源性较高。这些分析结果为蓖麻谷胱甘肽S-转移酶生物合成及其功能的进一步研究提供一定的理论与实践依据。
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| 关 键 词： | 蓖麻 谷胱甘肽S-转移酶 基因克隆 生物信息学分析 |
Gene Cloning and Bioinformatics Analysis of Castor GST |
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| Wen Qi,Liang Ta'na,Zhang Yanxin,Li Lili,Zhang Youjing,Huang Fenglan.Gene Cloning and Bioinformatics Analysis of Castor GST[J].Molecular Plant Breeding,2020(7):2161-2166. |
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| Authors: | Wen Qi Liang Ta'na Zhang Yanxin Li Lili Zhang Youjing Huang Fenglan |
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| Institution: | (College of Life Sciences and Food Engineering,Inner Mongolia University for the Nationalities,Tongliao,028000;Inner Mongolia Industrial Engi neering Research Center of University for Castor,Inner Mongolia Key Laboratory of Castor Breeding,Inner Mongolia Collaborate Innovation Center for Castor,Inner Mongolia University for the Nationalities,Tongliao,028000;Animal Husbandry and Veterinary Prairie Station,Urchin Township,Yakeshi,022159) |
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| Abstract: | Glutathione S-transferase(GST)is the key enzyme of glutathione binding reaction.It catalyzes the initial steps of glutathione binding reaction.It mainly exists in the cytosol and has many forms.It has certain pathological importance.In this study,GST gene was cloned and analyzed by bioinformatics.Polymerase chain reaction(PCR)was used to amplify the gene.The gene can encode 219 amino acids.The protein composed of 219 amino acids is a stable hydrophilic protein.There is an obvious signal peptide in the protein,in which alpha-helix and random coil are the main secondary structure.According to sequence homology alignment,castor,Jatropha curcas and durian clustered together,indicating that they had higher homology than other species.These results provide a theoretical and practical basis for further research on biosynthesis and function of castor glutathione S-transferase. |
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| Keywords: | Castor Glutathione S-transferase Gene cloning Bioinformatics analysis |
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