马蹄金(Dichondra repens Forst.)组织培养和快速繁殖

为了探究马蹄金组织培养及快繁技术,本研究以马蹄金为材料,使用75%的酒精和10%NaClO对不同外植体(胚根,下胚轴,子叶)进行消毒处理,在不同激素浓度配比的MS培养基中进行丛生芽诱导、愈伤组织诱导及分化,成功建立了马蹄金的组培快繁体系。结果表明:利用马蹄金下胚轴为外植体直接诱导不定芽效果较好,其最适程序为:流水冲洗30 min+75%酒精30 s+10%NaClO 9 min,污染率为5%,成活率为75%;下胚轴不定芽诱导的最适培养基为MS+1 mg/L 6-BA+0.3 mg/L NAA,诱导率为93.55%,出芽指数为8.86;胚根愈伤组织诱导的最适培养基为MS+1 mg/L 6-BA+0.5 mg/L NAA,诱导率为100%,下胚轴愈伤组织诱导的最适培养基为MS+0.5 mg/L 6-BA+1 mg/L NAA,诱导率为95.24%,子叶愈伤组织诱导的最适培养基为MS+0.3 mg/L 6-BA+0.5 mg/L NAA,诱导率为100%;愈伤组织不定芽分化的最适培养基为1/2MS+1.5 mg/L 6-BA+0.5 mg/L NAA,芽诱导率为20%,平均芽数为12.83;组培苗诱导生根的最适培养基为不加激素的1/2MS培养基;生根苗以瓶盖全开的炼苗方式移栽到以河沙为基质的育苗盒中成活率为100%,且添加蒸馏水与自来水对植株生长并无明显影响。本研究成功建立了马蹄金组织培养与快繁体系,为马蹄金种质资源改良及优质种苗生产提供了技术参考。

Tissue Culture and Rapid Propagation of Dichondra repens Forst.

To study the tissue culture and rapid propagation of different explants of Dichondra repens Forst..The radicle,hypocotyl and cotyledon of D.repens were used as explants,and 75%alcohol and 10%NaClO were used for disinfection.The effects of various hormone ratios on adventitious buds,callus inductions and differentiation were explored.Finally,a tissue culture and rapid propagation system of D.repens was established.The result showed that,it is best to directly induced adventitious buds by using the hypocotyls of D.repens.Rinsing with water for 30 min+75%alcohol 30 s+10%NaClO for 9 min as an optimum sterilization procedure,the contamination rate of explants was 5%and the survival rate to 75%.The optimal medium for adventitious bud induction from the hypocotyl was MS+1 mg/L 6-BA+0.3 mg/L NAA with an induction rate of 93.55%and the shoot forming index was 8.86.The optimum medium for radicle callus induction was MS+1 mg/L 6-BA+0.5 mg/L NAA with an induction rate to 100%.The optimum medium for hypocotyl callus induction was MS+0.5 mg/L 6-BA+1 mg/L NAA with an induction rate to 95.24%.The optimum medium for cotyledon callus induction was MS+0.3 mg/L 6-BA+0.5 mg/L NAA with an induction rate to 100%.The optimum medium for callus differentiation was 1/2MS+1.5 mg/L 6-BA+0.5 mg/L NAA with a shoot differentiation rate to 20%and the average number of adventitious buds was 12.83.The optimum medium for induction of rooting by tissue culture seedlings is 1/2MS without any hormones.The rooting seedlings were transplanted to the seedling box with river sand as the substrate,and the survival rate was up to 100%,and there was no significant difference in plant growth between distilled water and tap water.The study successfully established a rapid propagation system for the tissue culture of D.repens and provided a technical reference for the germplasm resources improvement and the production of high quality seedlings of D.repens.