翠竹及菲白竹LEA3同源基因的克隆及序列分析

竹类植物在中国有着非常悠久的栽培历史,并被广泛应用于园林、造林、庭院以及食品等行业。本研究以翠竹和菲白竹为材料提取其叶片总DNA,采用PCR方法获得翠竹SpLEA3基因与菲白竹SfLEA3-1、SfLEA3-2基因;其中SpLEA3全长804 bp,编码195个氨基酸,GC含量为68.4%;SfLEA3-1全长803 bp,编码195个氨基酸,GC含量为68.2%;SfLEA3-2全长557 bp,编码144个氨基酸,GC含量为67.8%。通过ProtParamy等生物软件分析,SpLEA3、SfLEA3-1和SfLEA3-2与贵州悬竹LEA3基因同源性高达61%以上,且3个基因编码蛋白均属于亲水性蛋白。源自2个竹种LEA3基因均包含一个完整的开放阅读框,其编码的氨基酸含有4~6个由11个氨基酸组成的保守基元序列。本研究不仅为深入了解竹类植物抗旱的分子机理研究提供了基础数据,也为竹类植物的抗旱育种后续研究提供了科学依据。

Cloning and Sequence Analysis of LEA3 Homologous Genes from Sasa pygmaea and Sasa fortunei

In China, bamboos have a very long cultivation history. They are widely used in many areas including gardening, afforestation, courtyard and food etc. In this study, the total DNA were extracted from the leaves of Sasa pygmaea and Sasa fortunei, SpLEA3 was amplified from S. pygmaea by PCR method while SfLEA3-1 and SfLEA3-2 from S. fortunei. Among them, the length of SpLEA3 was 804 bp, which encoded 195 amino acids with GC content of 68.4%;the length of SfLEA3-1 was 803 bp, encoded 195 amino acids with GC content of 68.2%,and the length of SfLEA3-2 was 557 bp, encoded 144 amino acids with GC content of 67.8%. Based on the analysis of three genes by using ProtParamy and other biosoftware, it showed that the homology between SpLEA3,SfLEA3-1, SfLEA3-2 and the LEA3 gene from Ampelocalamus calcareous was more than 61%, all the proteins of three genes belonged to hydrophilic ones, and all the three genes from two bamboos contained a complete open reading frame, whose amino acids possessed 4~6 conserved motif sequences which consisted of 11 amino acids.The results of this study was helpful not only to understand the molecular mechanisms of bamboo drought-resistance, but also to provide scientific basis for drought-resistance breeding of bamboos.