| 萝卜扩展蛋白基因RsEXPB1克隆与表达特征分析 |
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| 引用本文： | 荆赞革,柳李旺,龚义勤,姜立娜,孙新菊,周艳孔.萝卜扩展蛋白基因RsEXPB1克隆与表达特征分析[J].分子植物育种,2009,7(4):801-805. |
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| 作者姓名： | 荆赞革 柳李旺 龚义勤 姜立娜 孙新菊 周艳孔 |
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| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室,农业部南方蔬菜遗传改良重点开放实验室,南京,210095 |
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| 基金项目： | 国家自然科学基金项目，国家科技支撑计划课题，国家863计划 |
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| 摘 要： | 本文根据GenBank中扩展蛋白序列设计特异引物,从萝卜高代自交系"NAU-LVYH06"中克隆到一个扩展蛋白基因RsEXPB1(GenBank accession No.GQ387363,GQ387364).其编码区基因组序列长度为1 430bp,包括4个外显子和3个内含子;RT-PCR获得长度为898 bp的cDNA,开放读码框(ORF)为29～820 bp,推导编码263个氨基酸,含有1个组氨酸(His-Phe-Asp,HrD)功能域,ORF和推导蛋白序列与拟南芥AtEXPB3(GenBank accession No.NM118965)同源性分别为87%、92%.半定量RT-PCR检测到该基因主要在萝卜生育前期的幼叶、肉质根木质部和韧皮部中表达,且表达丰度可能与该部位细胞生长分裂状态相关,差异较明显.
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| 关 键 词： | 萝卜(Raphanus sativus L.) 扩展蛋白 基因克隆 表达特征 |
Cloning and Expression Characterization of the Expansin Gene RsEXPB1 in Radish |
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| Jing Zange,Liu Liwang,Gong Yiqin,Jiang Lina,Sun Xinju,Zhou Yankong.Cloning and Expression Characterization of the Expansin Gene RsEXPB1 in Radish[J].Molecular Plant Breeding,2009,7(4):801-805. |
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| Authors: | Jing Zange Liu Liwang Gong Yiqin Jiang Lina Sun Xinju Zhou Yankong |
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| Institution: | Jing Zange Liu Liwang Gong Yiqin Jiang Lina Sun Xinju Zhou Yankong National Key Laboratory of Crop Genetics , Germplasm Enhancement,Nanjing Agricultural University,Key Laboratory of Southern Vegetable Crop Genetic Improvement,Ministry of Agriculture,Nanjing,210095 |
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| Abstract: | Using the specific primers derived from the sequence of expansins in GenBank, the RsEXPB1 gene (GenBank accession No. GQ387363, GQ387364) was cloned from radish advanced inbred line NAU-LVYH06 in this study. The genome sequence of coding region was 1 430 bp including four exons and three introns. The cDNA consisted of 898 bp with an open reading frame (ORF) between 29 bp and 820 bp by RT-PCR, and the predicted protein of 263 amino acids, residues contained histidine function domain (His-Phe-Asp, HFD). The s... |
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