| 陆地棉异质型ACCase基因的种子特异表达载体构建与遗传转化 |
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| 引用本文： | 刘正杰,张园,王彦霞,李朋波,苏莹,张曦,王玉美,华金平.陆地棉异质型ACCase基因的种子特异表达载体构建与遗传转化[J].分子植物育种,2011,9(3):270-277. |
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| 作者姓名： | 刘正杰 张园 王彦霞 李朋波 苏莹 张曦 王玉美 华金平 |
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| 作者单位： | 中国农业大学农学与生物技术学院;湖北省农科院经济作物研究所; |
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| 基金项目： | 教育部新世纪优秀人才支持计划项目(NCET-06-0106); 国家自然科学基金项目(30871563); 国家转基因生物新品种培育重大专项课题(2009ZX08005-024B)共同资助 |
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| 摘 要： | 异质型ACCase催化乙酞CoA形成丙二酞辅酶A是植物脂肪酸合成途径中的第一个关键的步骤,也是限速步骤:植物体内过量表达ACCase能够增加脂肪酸合成的底物,有可能导致植株种子含油量的增加.本实验采用GUS基因瞬时表达检测棉花愈伤组织中种子特异性表达启动子AGP的活性;利用RT-PCR扩增,克隆了陆地棉异质型ACCas...
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| 关 键 词： | ACCase 种子特异启动子 载体构建 遗传转化 陆地棉 |
Construction of Seed Specific Expression Vectors and Genetic Transformation for Genes of Heteromeric ACCase in Upland Cotton |
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| Liu Zhengjie,Zhang Yuan,Wang Yanxia,Li Pengbo,Su Ying,Zhang Xi,Wang Yumei,Hua Jinping.Construction of Seed Specific Expression Vectors and Genetic Transformation for Genes of Heteromeric ACCase in Upland Cotton[J].Molecular Plant Breeding,2011,9(3):270-277. |
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| Authors: | Liu Zhengjie Zhang Yuan Wang Yanxia Li Pengbo Su Ying Zhang Xi Wang Yumei Hua Jinping |
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| Institution: | Liu Zhengjie 1 Zhang Yuan 1 Wang Yanxia 1 Li Pengbo 1 Su Ying 1 Zhang Xi 1 Wang Yumei 2 Hua Jinping 1 1 College of Agronomy & Biotechnology,China Agricultural University,Beijing,100193,2 Institute of Cash Crops,Hubei Academy of Agricultural Sciences,Wuhan,430064 |
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| Abstract: | The process of plastidial heteromeric ACCase isozyme catalyzes the formation of malonyl-CoA is the first and limiting step in fatty acid synthase of plant. over-expression of the ACCase would be a good strategy to manipulate for increasing seed oil content. GUS transient expression in cotton embryonic callus proved the activity of the seed specific expression promoter. Genes (BCCP1, BC1, CTa2 and CTb) encode the four subunits of heteromeric ACCase isozyme were obtained by RT-PCR, use the gene fusion strateg... |
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| Keywords: | ACCase Seed specific expression promoter Vector construction Transformation Upland cotton |
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