| 水稻光温敏雄性核不育系45S不育基因的分子定位 |
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| 引用本文： | 赵明富,蔡春苗,车容会,徐先战,郑建华,方珊如,吴春珠,施碧红.水稻光温敏雄性核不育系45S不育基因的分子定位[J].分子植物育种,2008,6(6). |
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| 作者姓名： | 赵明富 蔡春苗 车容会 徐先战 郑建华 方珊如 吴春珠 施碧红 |
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| 作者单位： | 1. 福建省农业科学院水稻研究所,福州,350018
2. 福建师范大学生命科学学院,福州,350018 |
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| 基金项目： | 福建省科技厅科研项目
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国家引进国际先进农业科学技术计划(948计划)
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| 摘 要： | 水稻光温敏雄性核不育系45S的育性转换受光长和温度的互补作用调控。对45S（Oryza sativa L．ssp．indica）WC169组合的F2,BC1F1和F2和BC2F2进行不育基因的遗传分析，结果表明45S的育性是由1对隐性不育基因控制。利用SSR分子标记技术，结合BSA和RCA法，以45S与YC169的杂交F2和BC2F2作为分离群体，对不育基因进行定位，结果发现不育基因（暂命名为GTMS）位于RM6378和RM12847之间，遗传距离分别为8．5cM和8．2cM。
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| 关 键 词： | 水稻 光温敏雄性核不育基因 分子定位 |
Molecular Mapping of Photoperiod-therom Sensitive Male Sterile Gene in Rice 45S |
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| Zhao Mingfu,Cai Chunmiao,Che Ronghui,Xu Xianzhan,Zheng Jianhua,Fang Shanru,Wu Chunzhu,Shi Bihong.Molecular Mapping of Photoperiod-therom Sensitive Male Sterile Gene in Rice 45S[J].Molecular Plant Breeding,2008,6(6). |
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| Authors: | Zhao Mingfu Cai Chunmiao Che Ronghui Xu Xianzhan Zheng Jianhua Fang Shanru Wu Chunzhu Shi Bihong |
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| Institution: | Zhao Mingfu 1* Cai Chunmiao 2 Che Ronghui 2 Xu Xianzhan 2 ZhengJianhua 1 FangShanru 1 Wu Chunzhu 1 Shi Bihong 2* 1 Institute of Rice,Fujian Academy of Agricultural Sciences,Fuzhou,350018,2 College of Life Sciences,Fujian Normal University |
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| Abstract: | The change of fertility for 45S, a photoperiod-thermo sensitive genic male sterile (P-(T)GMS) rice line, is regulated by complementary action of day-length and temperature changes. Genetic analysis indicated that the sterility of MS8S was controlled by a single recessive gene using F2, BC1F1, F2 and BC2F2 populations derived from the cross of 45S and YC169. A linkage analysis using molecular markers was performed in F2 and BC2F2 mapping population through bulked-segregant analysis (BSA) in combination with ... |
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