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| 三种改良提取长雄野生稻根叶总RNA方法的比较研究 | |
| 引用本文： | 林海妹,郭安平,王晓玲,郭运玲,孔华,贺立卡.三种改良提取长雄野生稻根叶总RNA方法的比较研究[J].分子植物育种,2009,7(1). |
| 作者姓名： | 林海妹 郭安平 王晓玲 郭运玲 孔华 贺立卡 |
| 作者单位： | 1. 海南大学,海口,570228;中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101 2. 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101 3. 江西省农业科学院水稻研究所,南昌,330200 |
| 基金项目： | 农业部农业结构调整重大技术研究专项项目，中央级公益性科研院所基本科研业务费专项基金，中央级公益性科研院所基本科研业务费专项基金 |
| 摘 要： | 为探讨野生稻总RNA的提取,笔者尝试了改良SDS、CTAB和TRIzol三种提取方法分别提取长雄野生稻根和叶总RNA,通过琼脂糖凝胶电泳和分光光度计检测,比较这三种方法的提取效果.结果表明,改良SDS、CTAB法提取的根总RNA的电泳条带清晰,几乎没有污染,无明显降解,OD260/OD280在1.75～2.0之间,能满足一般的分子操作要求,但所提取的叶片总RNA则有明显的DNA污染和少量的蛋白质污染.用TRJzol法提取的总RNA,无论是根还是叶片,均有不同程度蛋白质污染.若要进行下一步的分子操作,需先进行RNA的纯化,以去除蛋白质.可见改良SDS、CTAB法提取根的总RNA是首选. |
| 关 键 词： | 总RNA 长雄野生稻 提取方法 电泳 |
Comparison Study on three Modified Extracting Methods for Oryza Longisatminata Root and Leaves Total RNA |
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| Lin Haimei,Guo Anping,Wang Xiaoling,Guo Yunling,Kong Hua,He Lika.Comparison Study on three Modified Extracting Methods for Oryza Longisatminata Root and Leaves Total RNA[J].Molecular Plant Breeding,2009,7(1). | |
| Authors: | Lin Haimei Guo Anping Wang Xiaoling Guo Yunling Kong Hua He Lika |
| Institution: | Lin Haimei 1,2 Guo Anping 2 Wang Xiaoling 3 Guo Yunling 2 Kong Hua 2 He Lika 2 1 Hainan University,Haikou,570228,2 Institute of Tropical Bioscience , Biotechnology,Key Laboratory of Tropical Crop Biotechnology Ministry of Agricuiture,571101,3 Rice Research Institute,Jiangxi Academy of Agricultual Sciences,Nanchang,330200 |
| Abstract: | To explore the extraction of total RNA from Oryza longisatminata, the author attempt modified SDS method, CTAB method and TRIzol method, comparing the extraction effect of these methods, by electrophoresis in general agarose gel and spectrophotometer readings. The results indicates that modified SDS method, CTAB method for root RNA is good, its bonds of electrophores are clear, there are almost no contamination, no obvious degradation, the OD260/OD280 is between 1.75~2.0, it can meet the general equirements... |
| Keywords: | Total RNA Oryza longisatminata Extraction methods Electrophoresis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |