燕山板栗种质资源AFLP遗传多样性分析

为研究燕山板栗的遗传多样性,用扩增片段长度多态性(amplified fragment length polymorphism,AFLP)技术对44份板栗种质的遗传多样性及遗传距离进行分析,其中36份原产燕山地区的种质中有18份为野生种质和18份为栽培品种,另外8份为原产山东的品种.共检测了384个AFLP位点,有217个是多态性位点,占56.5%,这表明了所检测的板栗材料中存在着明显的遗传多样性.用NT.SYS软件对44份板栗种质进行聚类分析.所有材料聚成4个类群,其中燕山地区原产的板栗材料被聚为3个类群,山东起源的被聚类为1个独立的类群.在所分析的44份材料中,遗传距离在0.0292和0.2214之间.燕山板栗和外来板栗存在着明显的差异,本研究结果与本实验室用RAPD技术分析的结果是一致的.结果对这些板栗材料在育种中的应用具有重要指导意义.

Genetic Diversity of Chestnut Germplasm in Yanshan Region Revealed by AFLP

Genetic diversity of forty-four chestnuts including eighteen wild germplasms, eighteen cultivars originated from Yanshan region and eight cultivars collected from Shandong province were analysed using AFLP (amplifi ed fragment length polymorphism) approach, total of 384 loci of the chestnut genome were examined for molecular variation and 217 loci were polymorphism, which accounted for 56.5%. Clustering was conducted by using NTSYS software. The tested chestnuts can be divided into 4 groups. There was obvious difference between chestnuts originated from Yanshan and from Shandong. The genetic distance ranged from 0.0292 to 0.2214. The results showed in this research are quite similar with previous works revealed by RAPD in our lab and strongly implied that these data could be helpful in chestnut breeding program.