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水稻品种生殖生长期耐冷性及其低温胁迫下MADS-box基因表达差异分析
引用本文:冯德党,吕永刚,王国斌,甘树仙,张石来,张慧,徐家星,董陈文华,熊海波,朱骞,张小玲,魏振飞,李东宣,陈丽娟.水稻品种生殖生长期耐冷性及其低温胁迫下MADS-box基因表达差异分析[J].分子植物育种,2012(5):501-511.
作者姓名:冯德党  吕永刚  王国斌  甘树仙  张石来  张慧  徐家星  董陈文华  熊海波  朱骞  张小玲  魏振飞  李东宣  陈丽娟
作者单位:云南农业大学稻作研究所;云南省高校滇型杂交粳稻分子育种重点实验室
基金项目:NSFC-云南联合基金重点项目(U1136604);国家重点基础研究973项目(2011CB100401);云南省水稻产业技术体系(A3006516)共同资助
摘    要:选用来自中国不同稻作生态的4个特殊常规水稻品种:辐恢838(普通籼稻)、月亮谷(云南高海拔籼稻)、C418(普通粳稻)和丽粳11号(云南高寒粳稻),于减数分裂期和开花期分别低温(16℃)处理5d后又恢复5d,研究低温下生殖生长期的冷胁迫效应(花粉育性,结实率,百粒重下降程度)及相关MADS-box基因差异表达情况。结果表明:四个品种生殖生长期耐冷性由低到高排列依次是:辐恢838
关 键 词:水稻  籼粳  耐冷性  冷害  MADS-box基因

Differences in Cold Tolerance and Expression of MADS-box Genes under Low Temperature Stress in Various Rice Varieties at Reproductive Stage
Feng Dedang,Lv Yonggang,Wang Guobin,Gan Shuxian,Zhang Shilai,Zhang Hui,Xu Jiaxing,Dong Chenwenhua,Xiong Haibo,Zhu Qian,Zhang Xiaoling,Wei Zhenfei,Lee Dongsun, Chen Lijuan.Differences in Cold Tolerance and Expression of MADS-box Genes under Low Temperature Stress in Various Rice Varieties at Reproductive Stage[J].Molecular Plant Breeding,2012(5):501-511.
Authors:Feng Dedang  Lv Yonggang  Wang Guobin  Gan Shuxian  Zhang Shilai  Zhang Hui  Xu Jiaxing  Dong Chenwenhua  Xiong Haibo  Zhu Qian  Zhang Xiaoling  Wei Zhenfei  Lee Dongsun  Chen Lijuan
Institution:1,2* 1 Rice Research Institute, Yunnan Agricultural University, Kunming, 650201; 2 Key Lab of Molecular Breeding for Dian-Type Japonica Hybrid Rice of Yunnan Education Department, Kunming, 650201
Abstract:Four special conventional rice varieties from different ecological zones in China, namely Fuhui838 (common indica), Yuelianggu (conventional indica landrace growing at high altitude in Yunnan), C418 (common japonica) and Lijing11 (conventional japonica landrace growing in high altitude with cold climate in Yunnan), were used to study the cold tolerance at reproductive stage under low temperature stress. At reproductive stage (meiosis and flowering), the plants were treated at 16℃ for 5 days and then they were set to recover for 5 days under control. The pollen fertility, seed-setting rate and 100-grain weight were then measured, and the differential expression of MADS-box genes in rice was studied by RT-PCR. Results showed: the cold tolerance ranking from lower to higher were: Fuhui838
Keywords:Rice (Oryza sativa L  )  Indica and Japonica  Cold tolerance  Low temperature stress  MADS-box gene
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