割手密与甘蔗杂交F1群体构建及遗传多样性分子检测

为了获得更多割手密优良野生血缘的杂交后代,本研究利用割手密‘云南82-116’作为母本与父本‘桂糖05-3595’进行杂交。通过SSR引物MSSCIR1结合毛细管电泳技术,对48个杂交后代进行真假杂种鉴定,并分析遗传多样性。实验扩增出28个SSR片段,多态性条带比例67.9%,F1代均为真杂种。父母本及子代遗传相似系数在0.00~0.97之间,平均为0.58,后代遗传相似性整体较高,但遗传差异较大。UPGMA聚类分析显示,37份子代同父本聚为一类,遗传距离较近,遗传相似性较高。后期可将该结果用于指导杂交选育过程,提高育种效率,有效引入割手密的优质血缘。

Construction of F1Populations of S. spontaneum L. and Sugarcane and Molecular Detection of Genetic Diversity

In order to obtain more hyb rid offspring with excellent traits of Saccharum spontaneum L.,this research used the’YN82-116’as a female parent to hybridize with the male parent’GT05-3595’.The SSR primer MSSCIR1 combined with capillary electrophoresis technology was used to identify true and false hybrids of 48 hybrid progenies and to analyze genetic diversity.A total of 28 SSR fragments were amplified,the polymorphic bands ratio was 67.9%,and the F1 generations were all real hybrids.The parental and offspring inheritance similarity coefficients range from 0.00 to 0.97,with an average of 0.58.The overall genetic similarity of filial generation is high,but the genetic differences are large.UPGMA cluster analysis showed that 37 offspring were clustered with their father,with a closer genetic distance and higher genetic similarity.The results can be used in the later stage to guide the process of cross breeding,improve breeding efficiency,and effectively introduce germplasm resources that are densely S.spontaneum L..