311份小麦品种(系)优质麦谷蛋白亚基组成分析

为明确小麦品种(系)中优质麦谷蛋白亚基组成,筛选优质小麦品种资源,本研究以116份贵州省小麦品种(系)、195份省外小麦品种为供试材料,采用STS分子标记方法,对优质高分子量麦谷蛋白亚基Ax2^*、Bx7^oe、By8和低分子量麦谷蛋白亚基Glu-A3d、Glu-B3g进行分子检测,并利用近红外谷物品质分析仪测定311份材料的蛋白质含量,探讨了其与各个优质亚基间的相关性。结果表明:311份供试材料中,亚基Bx7oe、By8和Glu-B3g的分布频率分别为90%、78.14%和39.87%,优质亚基Ax2*和Glu-A3d分布较少,频率均为2.89%,不同优质麦谷蛋白亚基及组合对小麦蛋白质含量存在显著性影响,亚基Ax2^*和亚基组合7oe+8/A3d/B3g对蛋白质含量的贡献最大。本研究结果为改良小麦面筋质量、准确筛选优质种质资源提供依据。

Analysis of High-quality Glutenin Subunit Composition of 311 Wheat Varieties(Lines)

In order to determine the composition of high-quality glutenin subunits in wheat varieties(lines)and screen high-quality wheat varieties resources,116 wheat varieties(lines)from Guizhou Province and 195 wheat varieties from other areas were used as test materials.High molecular weight glutenin subunits Ax2^*,Bx7^oe,By8 and low molecular weight glutenin subunits Glu-A3d and Glu-B3g were detected by STS molecular markers.The main quality traits of 311 materials were determined by near infrared grain quality analyzer,and the correlation between protein content and each high quality subunit was discussed.The results showed that the frequencies of Bx7^oe,By8 and Glu-B3g were 90%,78.14%and 39.87%respectively in 311 tested materials,while Ax2^*and Glu-A3d were less distributed,and the frequencies were 2.89%.The results showed that different glutenin subunits and combinations had significant effects on wheat protein content,subunit Ax2^*and subunit combination 7oe+8/A3d/B3g had the largest contribution to protein content.The results provide a basis for improving the quality of wheat gluten and accurately screening high-quality germplasm resources.