三个大果型番茄花药愈伤组织诱导分析

本研究以3个大果型番茄杂交种F1代(‘1401’,‘1403’,‘卡莱’)的花药为试验材料,对其花药愈伤组织诱导的影响因素进行初步研究。结果表明:大果型番茄花药小孢子发育时期与花蕾长度以及花药长度呈正相关。较适番茄花蕾消毒方法为用70%酒精对番茄花蕾进行表面消毒30 s,然后再用加了0.1%吐温20的15%NaClO消毒7 min。3个供试番茄品种均能诱导产生愈伤组织,但不同番茄品种的愈伤组织诱导率不同。其中,‘1401’番茄的愈伤组织诱导率最高,为19.67%。诱导培养基中添加30 g/L蔗糖能促进愈伤组织的产生,生长素与细胞分裂素浓度比为1:2有利于愈伤组织的产生。较适愈伤组织诱导培养基为(murashige and skoog medium,MS)+0.5 mg/L IAA+1 mg/L 6-BA+30 g/L蔗糖+7 g/L琼脂粉。该研究为获得大果型番茄多倍体材料提供了技术支撑。

Anther Callus Induction Analysis of Three Large Fruit Tomato

The anthers of three large fruit tomato F1 hybrids(’1401’,’1403’,’Kale’)were used as experimental materials to preliminarily study the influencing factors of anther callus induction.The results showed that there was a positive correlation between the microspore developmental stage and the length of flower bud or anther.The appropriate sterilizing method of tomato flower bud was to surface-sterilize the tomato flower bud with 70%alcohol for 30 s,then sterilize it with 15%NaClO with 0.1%Tween 20 for 7 min.Callus could be induced from three tested tomato varieties,but the callus induction rate of different tomato varieties was different.Among them,’1401’tomato had the highest callus induction rate(19.67%).The induction medium with 30 g/L sucrose can promote the production of callus,the ratio of auxin to cytokinin was 1:2,which was beneficial to callus production.The appropriate medium for callus induction was MS+0.5 mg/L IAA+1 mg/L 6-BA+30 g/L sucrose+7 g/L agar powder.This study provided technical support for obtaining polyploidy materials of large fruit tomato.