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大豆遗传群体选择与品质QTL的获得
引用本文:魏崃,孙鸿雁,唐晓飞,杨喆,高明杰,刘丽君.大豆遗传群体选择与品质QTL的获得[J].分子植物育种,2009,7(4):727-735.
作者姓名:魏崃  孙鸿雁  唐晓飞  杨喆  高明杰  刘丽君
作者单位:1. 黑龙江省农业科学院大豆研究所,哈尔滨,150086;东北农业大学农学院,哈尔滨,150030
2. 黑龙江省农业科学院大豆研究所,哈尔滨,150086
基金项目:国家863项目,国家973项目 
摘    要:本文以高油品种哈交97-5404-1为母本,以哈交99-5448-4为父本,建立重组自交系群体.应用SSR技术,对不同世代F2∶3,F2∶6、F2∶9遗传群体中的油份QTL定位进行了分析,不同世代大豆分离遗传群体中油份含量均接近于正态分布,油份含量性状表达偏向于母本哈交97-5404-1,F2∶3代获得了对油份贡献率较高的QTL,F2∶9代则获多个与油份相关的QTL,大豆F2∶3和F2∶9代的遗传群体适宜用做品质性状的QTL定位,不同世代定位的油份QTL,均与SSR位点Satt193有关,通过对来自全国不同品种的SSR进行了分析和方差分析证实,Satt193在第一等位变异下(即DNA片段长度为270 bp)做为油份的筛选标记具有实用性,而在第三等位变异下(即DNA片段长度为220 bp)做为蛋白质材料的筛选标记具有应用性.

关 键 词:遗传群体  QTL定位  等位变异

Selection of Genetic Population and Obtaining of the Quality QTLs in Soybean
Wei Lai,Sun Hongyan,Tang Xiaofei,Yang Zhe,Gao Mingjie,Liu Lijun.Selection of Genetic Population and Obtaining of the Quality QTLs in Soybean[J].Molecular Plant Breeding,2009,7(4):727-735.
Authors:Wei Lai  Sun Hongyan  Tang Xiaofei  Yang Zhe  Gao Mingjie  Liu Lijun
Institution:Wei Lai 1,2 Sun Hongyan 1 Tang Xiaofei 1 Yang Zhe 1 Gao Mingjie 1 Liu Lijun 1 1 Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin,150086,2 College of Agronomy,Northeast Agriculture University,150030
Abstract:In this study, the RIL population was formed from a cross between high oil content cultivar hajiao975404-1 as female parent and Hajiao99-5448-4 as male parent. SSR technology was utilized, genetic maps were constructed and QTL tagging of oil content trait was also analyzed in three different generations F2:3, F2:6, F2:9. The oil content in the different generations was all close to normal distribution. The expression of the oil content trait in the population had trend to that of the female parent hajiao97-...
Keywords:Genetical population  QTL tagging  Equipotential line variation  
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