激素和低温预处理对胡麻花药愈伤组织诱导的影响

本文以胡麻杂交组合IS×95005的F_1为材料,研究了激素和低温预处理对花药愈伤组织诱导的影 响.研究结果表明,不同培养基不仅对花药愈伤组织的诱导率有影响,而且对愈伤组织的颜色、生长速率和质地也有较大的影响.KT+2,4-D激素组合对花药愈伤组织的诱导效果优于KT+NAA.接种前花蕾4℃低温预处理能显著提高化药愈伤诱导率.在相同培养基中,低温预处理大数对愈伤诱导率起主要作用.综合考虑愈伤组织的质量和诱导率,胡麻组合1S×95005的F_1代花药愈伤诱导率存低温预处理5 d,附加KT 1.0 mg/L+2,4-D2.0 mg/L的MS固体培养基上诱导效果最好.

Effects of Hormone and Low Temperature Pretreatment on Callus Induction of Oilflax

In this paper, we studied the effects of exogenous hormone and low temperature pretreatments on anther callus induction of oilflax (Linum usitatissimum L.). The results indicated that different mediums not only have influence on anther callus induction, but also greatly affect the color, growth rate and texture of callus. The effects of hormone combinations KT+2,4-D on anther callus induction are superior to that of hormone combinations KT+NAA.Lower temperature pretreatment of buds at 4℃ before inoculating significantly improved callus induction frequency.The days of low temperature pretreatment plays a dominant role in anther callus induction on the same culture medium. Comprehensively considering the callus quality and induction frequency, the optimal inducing conditions for anther callus of F_1 derived from the cross between oilflax 1S and 95005 are as follows: low temperature pretreatment for 5 days, MS solid medium containing 1.0 mg/L KT and 2.0 mg/L 2,4-D.