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Rf-1位点CAPS标记对水稻不同胞质雄性不育育性恢复系的关系分析
引用本文:谭亚玲,王石华,洪汝科,文建成,郭艾,寇淑燕,金寿林,谭学林.Rf-1位点CAPS标记对水稻不同胞质雄性不育育性恢复系的关系分析[J].分子植物育种,2009,7(3).
作者姓名:谭亚玲  王石华  洪汝科  文建成  郭艾  寇淑燕  金寿林  谭学林
作者单位:云南农业大学稻作研究所,昆明,650201
基金项目:教育部高等学校博士学科点专项科研基金,云南省科技厅科技攻关项目 
摘    要:水稻育性恢复基因Rf-1是目前水稻上克隆的唯一恢复基因.通过位于Rf-1位点的两个特异性CAPS(cleavable amplified polymorphic sequences)标记对滇I型、野败型、红莲型及BT型恢复系基因型分析,发现这四种不同胞质的恢复系在Rf-1位点具有相同的带型,滇I型的两个保持系具有不同的带型.结果表明这四种不同胞质系统的恢复系均具有Rf-1基因.该结论与从恢复系选育的系谱分析的结果相一致的.

关 键 词:水稻  育性恢复基因  细胞质雄性不育系  Rf-1位点  CAPS标记

Relationship among Fertility Restorers of Various CMS Systems Based on CAPS Markers Located at Rf-1 in Rice
Tan Yaling,Wan Shihua,Hong Ruke,Wen Jiancheng,Guo Ai,Kou Shuyan,Jin Shoulin,Tan Xuelin.Relationship among Fertility Restorers of Various CMS Systems Based on CAPS Markers Located at Rf-1 in Rice[J].Molecular Plant Breeding,2009,7(3).
Authors:Tan Yaling  Wan Shihua  Hong Ruke  Wen Jiancheng  Guo Ai  Kou Shuyan  Jin Shoulin  Tan Xuelin
Institution:Tan Yaling Wan Shihua Hong Ruke Wen Jiancheng Guo Ai Kou Shuyan Jin Shoulin Tan Xuelin Rice Research Institute of Yunnan Agricultural University,Kunming,650201
Abstract:Fertility restorers used in different hybrid rice based on cytoplasmic male sterility (CMS) of Dian-type-1 (DI), Wild abortive (WA), Hong-lian type (HL), and Boro-type (BT), were genotyped with two cleavable amplified polymorphic sequences (CAPS) markers located within Rf-1 gene, which is the only cloned fertility-restoring-gene in rice. The results showed that the restorers had the same DNA band pattern, but maintainer of CMS lines for CMS-D1 system had different band pattern. The results revealed that the...
Keywords:Rice  Fertility-restoring gene  Cytoplasmic male sterility  Rf-1  CAPS marker  
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