| 甘蔗常用亲本及优良组合双亲间遗传相似性分析 |
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| 引用本文： | 劳方业,刘睿,何慧怡,邓海华,李奇伟,陈仲华,陈健文,符成,齐永文,张垂明.甘蔗常用亲本及优良组合双亲间遗传相似性分析[J].分子植物育种,2009,7(3). |
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| 作者姓名： | 劳方业 刘睿 何慧怡 邓海华 李奇伟 陈仲华 陈健文 符成 齐永文 张垂明 |
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| 作者单位： | 广州甘蔗糖业研究所,广东省甘蔗改良与生物炼制重点实验室,广州,510316 |
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| 基金项目： | 广东省自然科学基金，广东省科技计划项目，农业部公益性行业(农业)科研专项基金，国家科技支撑计划 |
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| 摘 要： | 采用18对SSR引物和15对AFLP引物对我国196份甘蔗常用亲本的遗传相似性进行分析,分别获得438和1192个标记,多态性位点比例分别是87.60%,80.80%,平均为84.2%;亲本遗传相似系数变幅介于0.476～0.9099之间,总平均值为0.6673,遗传差异处于中等水平的亲本占了大多数,而差异较小和较大的都只是少数;45个甘蔗品种亲系的遗传相似系数分布在0.5687～0.6723,平均值为0.6723,91.11%甘蔗品种亲系的遗传相似系数集中在0.6100～0.7300之间,较理想的甘蔗杂交组合亲本间的遗传相似系数应为0.6723±0.0600;聚类分析表明,以遗传相似系数阈值约为0.700划分,育出45个品种所使用的30个亲本可分为9大类,在不同类型间选择亲本搭配的组合更容易选育出优良品种.
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| 关 键 词： | 甘蔗 遗传相似性 |
Analysis of Genetic Similarity among Commonly-Used Sugarcane Parents and between two Parents of Cross |
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| Lao Fangye,Liu Rui,He Huiyi,Deng Haihua,Li Qiwei,Chen Zhonghua,Chen Jianwen,Fu Cheng,Qi Yongwen,Zhang Chuiming.Analysis of Genetic Similarity among Commonly-Used Sugarcane Parents and between two Parents of Cross[J].Molecular Plant Breeding,2009,7(3). |
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| Authors: | Lao Fangye Liu Rui He Huiyi Deng Haihua Li Qiwei Chen Zhonghua Chen Jianwen Fu Cheng Qi Yongwen Zhang Chuiming |
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| Institution: | Lao Fangye Liu Rui He Huiyi Deng Haihua Li Qiwei Chen Zhonghua Chen Jianwen Fu Cheng Qi Yongwen Zhang Chuiming Guangzhou Sugarcane Industry Research Institute,Guangdong Key Lab of Sugarcane Improvement , Biorefinery,Guangzhou,510316 |
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| Abstract: | SSR and AFLP were employed to estimate genetic similarity among 196 sugarcane parents in common used. 18 SSR primer combinations produced a total of 438 bands, 87.60% of which were polymorphic, whereas 15 AFLP primer combinations produced a total 1 192 bands, 80.80% of which were polymorphic. The overall average rate of polymorphic loci was 84.2%. The genetic similarity index (GSI) among 196 parents estimated ranged from 0.476 1 to 0.909 9 with an average of 0.667 3. Most of the GSI between two parents were... |
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| Keywords: | SSR AFLP |
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