| 30个中国甘薯主栽品种的RAPD指纹图谱构建及遗传变异分析 |
| |
| 引用本文： | 王红意,翟红,王玉萍,何绍贞,刘庆昌.30个中国甘薯主栽品种的RAPD指纹图谱构建及遗传变异分析[J].分子植物育种,2009,7(5):879-884. |
| |
| 作者姓名： | 王红意 翟红 王玉萍 何绍贞 刘庆昌 |
| |
| 作者单位： | 中国农业大学农业部作物基因组学与遗传改良重点开放实验室,北京市作物遗传改良重点实验室,教育部作物杂种优势研究与利用重点实验室,北京,100193 |
| |
| 基金项目： | 现代甘薯产业技术体系，国家自然科学基金，农业行业计划 |
| |
| 摘 要： | 研究以中国30个甘薯主栽品种为材料,对甘薯RAPD指纹图谱的构建进行了探讨。从102个RAPD引物中筛选出26个多态性丰富的引物用于PCR扩增,共扩增出255条多态性条带,平均每个引物扩增的多态性带数为9.8条。其中S32和S39多态性最高,仅用其中1个引物即能将这30个甘薯品种完全区分开,且由此构建的指纹图谱出现的概率很小,分别为4.77×10^-7（1/221）和1.91×10^-6（1/219）。结果进一步表明,这30个甘薯品种的遗传距离变异幅度较大,为0.0390～0.4306,平均遗传距离为0.3086。RAPD聚类分析表明,地域分布相近的甘薯品种和具有同一亲本的甘薯品种聚在一起,与这些甘薯品种的系谱图一致。
|
| 关 键 词： | 甘薯（Ipomoea batatas（L.）Lam.） 主栽品种 RAPD指纹图谱 遗传变异 |
RAPD Fingerprints and Genetic Variations of the 30 Main Sweetpotato Varieties in China |
| |
| Wang Hongyi Zhai Hong Wang Yuping He Shaozhen Liu Qingchang Key Laboratory of Crop Genomics , Genetic Improvement,Ministry of Agriculture,Beijing Key Laboratory of Crop Genetic Improvement,Key Lab-oratory of Crop Heterosis , Utilization,Ministry of Education,China Agricultural University,Beijing.RAPD Fingerprints and Genetic Variations of the 30 Main Sweetpotato Varieties in China[J].Molecular Plant Breeding,2009,7(5):879-884. |
| |
| Authors: | Wang Hongyi Zhai Hong Wang Yuping He Shaozhen Liu Qingchang Key Laboratory of Crop Genomics Genetic Improvement Ministry of Agriculture Beijing Key Laboratory of Crop Genetic Improvement Key Lab-oratory of Crop Heterosis Utilization Ministry of Education China Agricultural University Beijing |
| |
| Institution: | Wang Hongyi Zhai Hong Wang Yuping He Shaozhen Liu Qingchang Key Laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture,Beijing Key Laboratory of Crop Genetic Improvement,Key Lab-oratory of Crop Heterosis and Utilization,Ministry of Education,China Agricultural University,Beijing,100193 |
| |
| Abstract: | The objectives of this study are to establish RAPD fingerprints of the 30 sweetpotato varieties widely planted in China.Twenty-six primers screened from 102 RAPD primers were polymorphic and generated 255 polymorphic bands with an average of 9.8 polymorphic bands per primer in the 30 sweetpotato varieties.Of these 26 RAPD primers,S32 and S39 were found to give the most polymorphic bands and clearly distinguished the 30 sweetpotato varieties,respectively,with the low value of DNA fingerprints constructed by ... |
| |
| Keywords: | Sweetpotato(Ipomoea batatas(L ) Lam ) Main variety RAPD fingerprint Genetic variation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
