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| 菜薹雄性不育相关基因的ISSR分子标记筛选 | |
| 引用本文： | 冒维维,高红胜,薄天岳,马金骏,徐东进,陈学好,贾志明,王永莉.菜薹雄性不育相关基因的ISSR分子标记筛选[J].分子植物育种,2009,7(1). |
| 作者姓名： | 冒维维 高红胜 薄天岳 马金骏 徐东进 陈学好 贾志明 王永莉 |
| 作者单位： | 1. 扬州大学园艺与植物保护学院,扬州,225009;江苏省姜堰主市农业局,泰州,225529 2. 扬州大学园艺与植物保护学院,扬州,225009 3. 上海市农业科学院园艺研究所,上海市设施园艺技术重点实验室,上海,201106 4. 扬州帮达蔬菜研究所,扬州,225009 |
| 基金项目： | 江苏省农业高新技术项目 |
| 摘 要： | 菜薹(Brassica campestris L.ssp.Chinensis var.utilis Tsenet Lee)属于十字花科芸薹属,原产中国,是华南地区重要的特产蔬菜之一.利用分子标记技术进行菜薹雄性不育相关基因的定位对菜蕞品质创新和选育新品种具有重要意义.目前尚未见有利用ISSR分子标记技术进行菜薹雄性不育分析的研究报道.利用ISSR技术对菜薹雄性不育系及其保持系的基因组DNA进行比较分析:结果显示100个ISSR引物中,只有引物UBC843的扩增结果在两系之间表现出了差异性,找到了不育系和保持系间的特异扩增片段.回收该特异扩增片段并进行克隆测序,测序结果表明该片段全长为462 bp.与白菜其它育性相关序列比较,同源性均低于50%.根据测序结果设计了一对特异引物,将其转化为更稳定的SCAR标记.经F2群体验证,ISSR引物扩增得到的特异片段很有可能来自核DNA. |
| 关 键 词： | 菜薹 雄性不育 分子标记 |
Identification of the Specfic ISSR Marker Linked to Male Sterility Gene of Brassica campestris L. ssp. Chinensis var. utilis Tsenet Lee |
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| Mao Weiwei,Gao Hongsheng,Bo Tianyue,Ma Jinjun,Xu Dongjin,Chen Xuehao,Jia Zhiming,Wang Yongli.Identification of the Specfic ISSR Marker Linked to Male Sterility Gene of Brassica campestris L. ssp. Chinensis var. utilis Tsenet Lee[J].Molecular Plant Breeding,2009,7(1). | |
| Authors: | Mao Weiwei Gao Hongsheng Bo Tianyue Ma Jinjun Xu Dongjin Chen Xuehao Jia Zhiming Wang Yongli |
| Institution: | Mao Weiwei 1,4 Gao Hongsheng 1 Bo Tianyue 2 Ma Jinjun 1 Xu Dongjin 3 Chen Xuehao 1 Jia Zhiming 3 Wang Yongli 3 1 School Horticulture , Plant Protection of Yangzhou University,Yangzhou,225009,2 Horticultural Research Institute,Shanghai Academic Sci- ences,Shanghai Key Lab of Protected Horticultural Technology,Shanghai,201106,3 Bangda Vegetable Research Institute of Yangzhou,4 Jiangyan Agricultural Bureau,Taizhou,225529 |
| Abstract: | Brassica campestris L. ssp. Chinensis var. utilis Tsen et Lee belongs to Cruciferae Brassica and is native to China, and is one of important vegetables mainly grown in the wide area of South China. Researches on male sterility gene of Brassica campestris L. ssp. Chinensis var. utilis with molecular makers play an important role in innovation of germplasmsa and breeding for new varieties. It has not been reported that ISSR is used to analysis male sterility gene of Brassica campestris L. ssp. Chinensis var. ... |
| Keywords: | Brassica campestris L ssp Chinensis var utilis Tsen et Lee Male sterility Molecular maker |
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