| 影响小白菜子叶再生的因素 |
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| 引用本文： | 赵慧,侯雷平,王慧,张学智,李梅兰.影响小白菜子叶再生的因素[J].分子植物育种,2009,7(5):972-977. |
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| 作者姓名： | 赵慧 侯雷平 王慧 张学智 李梅兰 |
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| 作者单位： | 山西农业大学园艺学院,太谷,030801 |
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| 基金项目： | 山西省留学回国人员科研资助项目 |
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| 摘 要： | 本研究以小白菜“杭州油冬儿”品种为试材,研究了不同激素组合、子叶不同部位、不同接种方式对不定芽再生的影响,并且还筛选了种子表面消毒的最佳方法和不定芽生根的最佳培养基。研究结果表明,白菜种子表面消毒的最佳方法为用70%的乙醇浸泡30s,再用20%的次氯酸钠消毒20min或用0.1%升汞消毒10min;以完整的带柄子叶为最佳外植体,接种时将子叶背面朝上放置比正面朝上放置更有利于不定芽的分化;子叶再生的最佳培养基为MN＋BA2mg/L＋NAA0.5mg/L,不定芽生根的最佳培养基为1/2MS＋IAA0.2mg/L。此研究为小白菜再生体系的建立奠定了基础。
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| 关 键 词： | 小白菜 子叶 诱愈 诱芽 |
Factors Affecting Plant Regeneration of Cotyledons of Chinese Cabbage |
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| Zhao Hui Hou Leiping Wang Hui Zhang Xuezhi Li Meilan College of Horticulture,Shanxi Agriculture University,Taigu.Factors Affecting Plant Regeneration of Cotyledons of Chinese Cabbage[J].Molecular Plant Breeding,2009,7(5):972-977. |
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| Authors: | Zhao Hui Hou Leiping Wang Hui Zhang Xuezhi Li Meilan College of Horticulture Shanxi Agriculture University Taigu |
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| Institution: | Zhao Hui Hou Leiping Wang Hui Zhang Xuezhi Li Meilan College of Horticulture,Shanxi Agriculture University,Taigu,030801 |
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| Abstract: | In this research,Hangzhou Youdongerwas used as experimental material to be studied for the regeneration of adventitious buds under the conditions of different hormone combination,different parts of cotyledons and cotyledon inoculation method.We have successfully found out the best method for seeds surface disinfection and the most appropriate medium for the rooting of adventitious buds.Our experiment showed that the best method for surface disinfection of Chinese cabbage seeds is as follows:first soak the s... |
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| Keywords: | Chinese cabbage Cotyledon Callus inducing Buds inducing |
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