| 火龙果茎腐病致病菌的鉴定 |
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| 引用本文： | 陈圆,严婉荣,肖敏,肖彤斌,赵志祥,王海洪.火龙果茎腐病致病菌的鉴定[J].分子植物育种,2019,17(3):904-909. |
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| 作者姓名： | 陈圆 严婉荣 肖敏 肖彤斌 赵志祥 王海洪 |
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| 作者单位： | 海南省农业科学院植物保护研究所,海南省植物病虫害防控重点实验室,农业农村部海口作物有害生物科学观测实验站,海口,571100;海南省农业科学院农业环境与土壤研究所,海南省耕地保育重点实验室,海口,571100 |
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| 基金项目： | 公益性行业(农业)科研专项;海南省科研院所技术研发专项;海南省科研院所技术研发专项 |
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| 摘 要： | 为弄清火龙果茎腐病的病原和分类地位,并进一步为该病害的有效防治提供理论依据。本研究从琼海、临高、陵水等地广泛采集病样。经田间症状调查、组织纯化培养、病原形态观察、致病力测定、真菌ITS序列和系统进化分析,鉴定了该病害的病原。结果表明:病原分离物具有典型的镰刀菌形态特征,菌丝浓密,产生紫色素,小型分生孢子新月形,大型分生孢子镰刀形,有多个隔;而在ITS序列分析中,代表菌株47A4和47A5与NCBI数据库中尖孢镰刀菌的多条ITS序列(登录号分别为KJ653447.1, DQ535184.1和KM268692.1)相似性达100%;系统进化树上,47A4和47A5与尖孢镰刀菌聚在同一分支上,遗传距离最近,而与非纯培养镰刀菌克隆3.91E在不同分支上,遗传距离远。结合形态特征、序列分析和系统进化分析,将火龙果茎腐病的病原鉴定为尖孢镰刀菌(Fusarium oxysporum)。研究结果对火龙果茎腐病流行规律、致灾机理的研究具有重要的指导作用。
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| 关 键 词： | 火龙果 茎腐病 病原鉴定 系统进化分析 |
Identification on Pathogenic Fungi of Dragon Fruit Stem Rot Disease |
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| Chen Yuan,Yan Wanrong,Xiao Min,Xiao Tongbin,Zhao Zhixiang,Wang Haihong.Identification on Pathogenic Fungi of Dragon Fruit Stem Rot Disease[J].Molecular Plant Breeding,2019,17(3):904-909. |
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| Authors: | Chen Yuan Yan Wanrong Xiao Min Xiao Tongbin Zhao Zhixiang Wang Haihong |
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| Institution: | (Scientific Observation and Experiment Station of Crop Pests in HaiKou,Ministry of Agriculture,Hainan Key Laboratory for Control of Plant Diseases and Insect Pests,Institute of Plant Protection of Hainan Academy of Agricultural Sciences,Haikou,571100;Hainan Key Laboratory of Arable Land Conservation,Institute of Agro-Environment and Soil of Hainan Academy of Agricultural Scienees,Haikou,571100) |
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| Abstract: | Chen Yuan;Yan Wanrong;Xiao Min;Xiao Tongbin;Zhao Zhixiang;Wang Haihong(Scientific Observation and Experiment Station of Crop Pests in HaiKou,Ministry of Agriculture,Hainan Key Laboratory for Control of Plant Diseases and Insect Pests,Institute of Plant Protection of Hainan Academy of Agricultural Sciences,Haikou,571100;Hainan Key Laboratory of Arable Land Conservation,Institute of Agro-Environment and Soil of Hainan Academy of Agricultural Scienees,Haikou,571100) |
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| Keywords: | Dragon fruit Stem rot disease Pathogenic identification Phylogenetic analysis |
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