| 抗稻瘟病基因Pi-2(t)紧密连锁的SSR标记的筛选与应用 |
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| 引用本文： | 陈志伟,郑燕,吴为人,赵长江.抗稻瘟病基因Pi-2(t)紧密连锁的SSR标记的筛选与应用[J].分子植物育种,2004,2(3):321-325. |
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| 作者姓名： | 陈志伟 郑燕 吴为人 赵长江 |
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| 作者单位： | 1. 福建农林大学作物科学学院,福州,350002
2. 福建农林大学植保学院,福州,350002 |
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| 摘 要： | 根据已报道的双侧连锁标记并利用已公布的水稻基因组序列信息，在广谱抗稻瘟病基因Pi-2（t)的附近找到了一个新的SSR标记，记为SRM24，估计它与Pi-2（t)间的距离大约只有0．5cM或43kb。利用该标记，成功地将Pi-2（t)从供体材料5173导入到迄今广泛使用的雄性不育保持系珍汕97B中，获得了一批携有Pi-2（t)的珍汕97B近等基因系。
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| 关 键 词： | 抗稻瘟病基因 Pi-2（t） SSR标记 筛选方法 水稻基因组 序列信息 |
Screening and Application of an SSR Marker Closely Linked to Pi-2(t),a Gene Resistant to Rice Blast |
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| Cheng Zhiwei Zheng Yan Wu Weiren Zhao Changjiang
College of Crop Science,Fujian Agriculture and Forestry University,Fuzhou,China
College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,China
Authors who made equal contributions to the work
Corresponding Author,zjweiren@pub.fz.fj.cn.Screening and Application of an SSR Marker Closely Linked to Pi-2(t),a Gene Resistant to Rice Blast[J].Molecular Plant Breeding,2004,2(3):321-325. |
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| Authors: | Cheng Zhiwei Zheng Yan Wu Weiren Zhao Changjiang
College of Crop Science Fujian Agriculture and Forestry University Fuzhou China
College of Plant Protection Fujian Agriculture and Forestry University Fuzhou China
Authors who made equal contributions to the work
Corresponding Author zjweiren@pubfzfjcn |
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| Institution: | Cheng Zhiwei Zheng Yan Wu Weiren Zhao Changjiang
College of Crop Science,Fujian Agriculture and Forestry University,Fuzhou,350002,China
College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,350002,China
Authors who made equal contributions to the work
Corresponding Author,zjweiren@pub5.fz.fj.cn |
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| Abstract: | According to the published information of flanking markers and rice genomic sequence, a new SSR marker, designated SRM24, was found near to Pi-2(t), a gene resistant to rice blast with wide spectrum. It was estimated that the marker is only about 0.5 cM or 43 kb away from Pi-2(t). With the marker, Pi-2(t) was successfully intro-gressed from a donor 5173 into Zhenshan 97B, a male sterile maintainer line most widely used to date, and a batch of isogenic lines of Zhenshan 97B carrying Pi-2(t) were created. |
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| Keywords: | Rice blast Pi-2(t) SSR SRM24 |
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