| 水稻外源DNA导入系的创建及主要性状分析 |
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| 引用本文： | 王丰,柳武革,李金华,刘振荣,李曙光,廖亦龙,彭惠普,毛兴学.水稻外源DNA导入系的创建及主要性状分析[J].分子植物育种,2004,2(4):489-494. |
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| 作者姓名： | 王丰 柳武革 李金华 刘振荣 李曙光 廖亦龙 彭惠普 毛兴学 |
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| 作者单位： | 广东省农科院水稻研究所,广州,510640;广东省农科院水稻研究所,广州,510640;江西农业大学农学院,南昌,330045;广东省农科院水稻研究所,广州,510640;农业部水稻遗传改良重点开放实验室,广东省水稻育种新技术重点实验室,广州,510640 |
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| 基金项目： | 本研究得到国家863计划项目(2001AA211161)、广东省农业科技重大专项(2001A2010101)的资助. |
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| 摘 要： | 通过花粉管通道法将稗草总体DNA和玉米总体DNA导入水稻受体R122中，获得的D1代变异频率分别高达1．487％和3．81％。对稳定的3个玉米DNA导入系和4个稗草DNA导入系研究表明，与受体R122相比，导入系在株型、株高、生育期、分蘖力、着粒密度、粒形、稃尖颜色、稻瘟病抗性、稻米品质、恢复力和耐储藏性等生物学性状方面发生了广泛的变异。同时讨论了外源DNA导入水稻的变异频率和在水稻育种中的应用前景。
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| 关 键 词： | 外源DNA导入 水稻 导入系 生物学性状 变异 |
Construction of Exogenous DNA Introgression Lines and Analysis of its Main Traits in Rice |
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| Wang Feng # Liu Wuge# Li Jinhua, Liu Zhenrong Li Shuguang Liao Yilong Peng Huipu Mao Xingxue, Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou, College of Agronomy,Jiangxi Agricultural University,Nanchang, Key Laboratory of Rice Genetics Improvement,Ministry of Agriculture, Rice Breeding New Technology Key Laboratory of Guangdong,Guangzhou.Construction of Exogenous DNA Introgression Lines and Analysis of its Main Traits in Rice[J].Molecular Plant Breeding,2004,2(4):489-494. |
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| Authors: | Wang Feng # Liu Wuge# Li Jinhua Liu Zhenrong Li Shuguang Liao Yilong Peng Huipu Mao Xingxue Rice Research Institute Guangdong Academy of Agricultural Sciences Guangzhou College of Agronomy Jiangxi Agricultural University Nanchang Key Laboratory of Rice Genetics Improvement Ministry of Agriculture Rice Breeding New Technology Key Laboratory of Guangdong Guangzhou |
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| Institution: | Wang Feng1 #* Liu Wuge1# Li Jinhua1,2 Liu Zhenrong1 Li Shuguang1 Liao Yilong1 Peng Huipu1 Mao Xingxue1,31 Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,5106402 College of Agronomy,Jiangxi Agricultural University,Nanchang,3300453 Key Laboratory of Rice Genetics Improvement,Ministry of Agriculture, Rice Breeding New Technology Key Laboratory of Guangdong,Guangzhou,510640 |
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| Abstract: | The direct introduction of Maize (Zea Mays L.) DNA and barnyard grass (Echinochloa crusgalli) DNA into rice recipient R122 were carried out through pollen-tube-pathway method and the frequency of variation in D1 were 1.487% and 3.81%, respectively. Four stable maize DNA introgression lines and 4 stable barnyard grass DNA introgression lines were constructed and then investigated. The results suggested that, the introgression lines showed extensive changes, compared with the recipient R122, in biological traits, such as plant type, plant height, growth duration, tillering ability, spikelet setting density, grain shape, apiculus color, blast resistance, rice quality, restoring ability and storability characters. The frequency of variation and utilization of exogenous DNA introduction in rice breeding were also discussed. |
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| Keywords: | Exogenous DNA introduction Rice Introgression lines Biological traits Variation |
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