DNA amplification fingerprinting and marker screening for pseudo-testcross mapping of flowering dogwood ( Cornus florida L.) |
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Authors: | G Caetano-anollés SE Schlarbaum RN Trigiano |
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Institution: | (1) Department of Biology, University of Oslo, Blindern, 0316 Oslo, Norway;(2) Department of Forestry, Wildlife and Fisheries, Tennessee Agricultural Experiment Station, The University of Tennessee, Knoxville, Tennessee, 37901–1701, U.S.A.;(3) Department of Ornamental Horticulture and Landscape Design, Tennessee Agricultural Experiment Station, The University of Tennessee, Knoxville, Tennessee, 37901–1701, U.S.A |
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Abstract: | DNA amplification fingerprinting (DAF) with arbitrary oligonucleotide primers was used to study genetic relationships between
cultivars of flowering dogwood (Cornus florida L.), evaluate extent of plant hybridization, and generate markers in pseudo-testcross
mapping at the intraspecific level. Modified Taguchi optimization methods defined a robust DAF system based on high annealing
temperature (48–52 °C) and primer concentration (typically 8 μM) that was used to study genetic diversity of representative
dogwood cultivars and hybrids. Phenetic analysis using cluster and numerical methods showed that: (1) cultivars were relatively
conserved at the genetic level; (2) their hybridization could be identified in the F1 progeny in the absence of phenotypic
or physiological markers; (3) several cultivars grouped according to their recorded ancestry; and (4) dogwood anthracnose-resistant
lines originally selected in Catoctin Mountain Park (Maryland) grouped separately from those of southern origin. The DAF protocol
was also tested in pseudo-testcross mapping of dogwood at the intraspecific level. A preliminary screening of parents ‘Pink
Sachet’ and ‘Fragrant Cloud’ and 7 F1 segregants with 22 octamer primers produced 703 amplified loci, 30 and 39 of which were
male and female markers segregating at 1:1 ratios with 98.6% confidence levels in pseudo-testcross configuration. Overall
results show that DAF generated markers very efficiently (3 per primer) despite the close relatedness of parental dogwood
cultivars. This study constitutes the basis for a future genetic linkage mapping and marker-assisted selection (MAS) effort
initially targeted to control important fungal diseases in dogwood.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | arbitrary primers Cornus florida L cultivar identification DNA amplification fingerprinting (DAF) dogwood pseudo-testcross Taguchi |
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