Genetic diversity of soybean germplasm resistant to Heterodera glycines |
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Authors: | J Zhang PR Arelli DA Sleper BX Qiu MR Ellersieck |
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Institution: | (1) Department of Agronomy, University of Missouri-Columbia, Columbia, MO, 65211, U.S.A;(2) Department of Statistics, University of Missouri-Columbia, Columbia, MO, 65211, U.S.A |
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Abstract: | Information on the genetic background of soybean Glycine max (L.) Merr.] germplasm resistant to Soybean Cyst Nematode (SCN)
(Heterodera glycines Ichinohe) is essential for developing plant breeding strategies, however, pedigree information for SCN
resistant germplasm is not always available. Using restriction fragment length polymorphisms (RFLPs) to fingerprint different
soybean lines is a useful means of determining genetic relationships and identifying unique genotypes. In this study, 102
soybean genomic probes with five different restriction enzymes were used to detect DNA variations and investigate genetic
relationships among 56 soybean plant introductions (PIs) and cultivars. Among 510 probe/enzyme combinations, a total of 1707
hybridization bands were generated, of which 501 were polymorphic. A cluster dendrogram and a principal component analysis
(PCA) plot diagram were constructed using DNA fingerprinting of the 56 soybean lines. Based on the clustering and PCA analyses,
two major clusters comprising 15 groups were identified for the PIs and cultivars in the dendrogram. Reactions to different
race isolates of SCN were distinguishable among different groupings of the clusters and PCA results, and various origins of
the PIs and the pedigree information of the cultivars could be associated with the different clusters. Additional information
on the appropriate number of probes used to detect genetic diversity more efficiently was elucidated through this research.
We believe that the genetic relationships determined among these 56 soybean lines could provide useful information in identifying
unique sources of genes for SCN resistance.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | cluster analysis genetic diversity Heterodera glycines PCA-RFLP |
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