Progenies of allotriploids of Oriental × Asiatic lilies (Lilium) examined by GISH analysis |
| |
Authors: | Rodrigo Barba-Gonzalez Alex A Van Silfhout Richard G F Visser Munikote S Ramanna Jaap M Van Tuyl |
| |
Institution: | 1. Dise?o del Estado de Jalisco, A.C. Biotecnología Vegetal, Centro de Investigación y Asistencia en Tecnología y, Av. Normalistas #800, Colinas de la Normal, Guadalajara, Jalisco, C.P., 44270, México 2. Plant Research International, Wageningen University and Research Centre, P.O. Box 16, 6700 AA, Wageningen, The Netherlands 3. Graduate School of Experimental Plant Sciences, Laboratory of Plant Breeding, Wageningen University and Research Centre, P.O. Box 386, 6700, Wageningen, AJ, The Netherlands
|
| |
Abstract: | With the aim of utilizing allotriploid (2n = 3x = 36) lily hybrids (Lilium) in introgression breeding, different types of crosses were made. First, using diploid Asiatic lilies (2n = 2x = 24), reciprocal crosses (3x − 2x and 2x − 3x) were made with allotriploid hybrids (AOA) obtained by backcrosses of F1 Oriental × Asiatic hybrids (OA) to Asiatic cultivars (A). Secondly, the AOA allotriploids were crossed with allotetraploid (OAOA, 2n = 4x = 48), in 3x − 4x combination. Finally, the AOA allotriploids where crossed to 2n gamete producer F1 OA hybrids (3x − 2x (2n)). Two types of triploids were used as parents in the different types of crosses, derived from: (a) mitotic polyploidization and (b) sexual polyploidization. Ploidy level of the progeny was determined by estimating the DNA values through flowcytometry as well as chromosome counting. The aneuploid progeny plants from 3x − 2x and reciprocal crosses had approximate diploid levels and in 3x − 4x crosses and 3x − 2x (2n) the progeny had approximate tetraploid levels. Balanced euploid gametes (x, 2x and 3x) were formed in the AOA genotypes. Recombinant chromosomes were found in the progenies of all crosses, except in the case of 2x − 3x crosses through genomic in situ hybridization (GISH) analyses. Recombinant chromosomes occurred in the F1 OA hybrid when the triploid AOA hybrid was derived through sexual polyploidization, but not through mitotic polyploidization with two exceptions. Those recombinant chromosomes were transmitted to the progenies in variable frequencies. |
| |
Keywords: | 2n gametes Allotriploids Genomic in situ
hybridization Lilium Poliploidization |
本文献已被 SpringerLink 等数据库收录! |
|