Analysis of wheat-Thinopyrum intermedium derivatives with BYDV-resistance |
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| Authors: | Zhi-Shan Lin Zhi-Fu Cui Xiang-Yan Zeng You-Zhi Ma Zeng-Yan Zhang Toshiki Nakamura Goro Ishikawa Kazuhiro Nakamura Hisashi Yoshida Zhi-Yong Xin |
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| Institution: | (1) National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100081, P.R. China;(2) National Agricultural Research Centre for Tohoku Region, 4 Akahira, Shimo-kuriyagawa, Morioka Iwate, 020-0198, Japan;(3) Japan International Cooperation Agency (JICA), Japan-China Research and Development Centre for Agricultural Technology, CAAS, Beijing, 100081, P.R. China |
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| Abstract: | Chromosome compositions of seven lines, derived from hybrids between a wheat cultivar and the wheat-Thinopyrum intermedium addition line Z6, with barley yellow dwarf virus (BYDV) resistance, were determined by genomic in situ hybridization, cytogenetic and SSR assays. The results showed that
line N522 was a disomic addition line, lines N420 and N439 were 2Ai-2(2B) chromosome substitution lines, lines N431 and N452
were 2Ai-2(2D) chromosome substitution lines, line N523 was a 2Ai-2S(2D) ditelosomic substitution line, and line N530 was
a double ditelosomic line with the mitotic chromosome number of 2n = 40 + 4t. One pair of telosomes in line N530 lacked several proximal SSR markers of chromosome 2AS, but possessed certain
terminal markers, which were consistent with an acrocentric structure, and the other pair of chromosome arms were presumably
2Ai-2S telosomes with BYDV-resistance. These wheat-Th. intermedium lines provide useful genetic resources for developing alien chromosome translocation lines. |
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| Keywords: | Chromosome addition line BYDV Cytogenetics GISH SSR Chromosome substitution line |
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