Development of SCAR and CAPS markers linked to a recessive male sterility gene in lettuce (<Emphasis Type="Italic">Lactuca sativa</Emphasis> L.) |
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Authors: | M Hayashi A Ujiie H Serizawa H Sassa H Kakui T Oda T Koba |
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Institution: | (1) Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo City Chiba, 271-8510, Japan;(2) Nagano Vegetable and Ornamental Crops Experimental Station, 1066-1 Aza Tokoo, Oaza Souga, Siojiri City Nagano, 399-6461, Japan; |
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Abstract: | Genetic male sterility (GMS) has been a useful system for the production of hybrid varieties in self-pollinated plants. We
obtained a GMS line developed from a spontaneous mutation in lettuce (Lactuca sativa L.). Genetic analysis in our previous study revealed that the sterility was controlled by a recessive gene which was named
ms-S. For simple and quick screening of individuals showing male sterility, we attempted molecular mapping of the ms-S locus using an amplified fragment length polymorphism (AFLP) technique. From the examination of 4,096 AFLP primer combinations,
63 AFLP markers were found to be linked to the gene and nine of them were successfully converted into sequence characterized
amplified region (SCAR) markers and cleaved amplified polymorphic sequence (CAPS) markers. Linkage analysis indicated that
these nine markers were closely linked to the ms-S gene and all were located on the same side of the gene. The minimum genetic distance between the ms-S gene and a marker was 3.1 cM. These results provide additional information for map-based cloning of the ms-S gene and will be of great help for lettuce breeding using GMS to produce F1 hybrids. |
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