Genetic variation and cultivar identification of Jamaican yam germplasm by random amplified polymorphic DNA analysis |
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| Authors: | H N Asemota J Ramser C Lopéz-Peralta K Weising G Kahl |
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| Institution: | (1) Biotechnology Centre, University of the West Indies, 7 Mona, Kingston, Jamaica;(2) Biochemistry Department, University of the West Indies, 7 Mona, Kingston, Jamaica;(3) Plant Molecular Biology, Biozentrum, Frankfurt University, 60439 Frandfurt/Main, Germany;(4) Colegio de Postgraduados, Carr. Mexico-Texcoco, 56230 Montecillo, Texcoco Edo, Mexico;(5) Present address: Biochemistry Department, University of Benin, PMB 1154 Benin City, Nigeria |
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| Abstract: | Summary We have used random amplified polymorphic DNA (RAPD) analysis to characterize eleven cultivars of the five economically most important yam species grown in Jamaica (Dioscorea alata, D. cayenensis, D. rotundata, D. trifida and D. esculenta). Amplification of genomic DNA samples with nine different arbitrary 10mer primers revealed a total of 338 different band positions, ranging in size from 0.3 to 2.5 kb. RAPD patterns proved to be highly reproducible and somatically stable. While no variation was observed among plants belonging to the same cultivar, a large number of intervarietal and interspecific polymorphisms enabled us to reliably discriminate between all Jamaican cultivars investigated. |
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| Keywords: | Dioscorea yam RAPD analysis genetic variation cultivar identification |
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