Parameters influencing the regeneration capacity of calluses derived from mature indica and japonica rice seeds after microprojectile bombardment |
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Authors: | Julio Alfonso-rubí Pilar Carbonero Isabel Díaz |
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Institution: | (1) Centro de Ingeniería Genética y Biotecnología, Sancti Spiritus, Apartado 83, 60200, Cuba;(2) Laboratorio de Bioquímica y Biología Molecular. Departamento de Biotecnología-UPM, Escuela Técnica Superior de Ingenieros Agrónomos, 28040 Madrid, Spain |
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Abstract: | The microprojectile bombardment procedure has allowed the stable transformation of indica and japonica rice varieties, although
at different frequencies of transformation depending mainly on their regeneration capacity and on the specific parameters
of the transformation protocol. A study of the process of regeneration to whole plants from primary calli derived from mature
indica and japonica rice seeds, via embryogenesis, has shown that somatic embryos are produced by division and differentiation
of the external cell layers of callus tissues. Adjusting the bombardment conditions to optimize gene delivery to those regenerable
cells, we have evaluated the influence of parameters such as the target distance, particle penetration and the effect of osmotic
treatment on the regeneration capacity of bombarded cells.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | gus expressing cells histological analysis japonica and indica rice particle bombardment primary callus regeneration capacity |
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