Molecular markers linked to the <Emphasis Type="Italic">fin</Emphasis> gene controlling determinate growth habit in common bean |
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Authors: | Astrid Pañeda Cristina Rodríguez-Suárez Ana Campa Juan José Ferreira Ramón Giraldez |
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Institution: | (1) State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, Shandong, 271018, P.R. China;(2) Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, 350013, P.R. China |
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Abstract: | The S-genotypes of 16 apricot (Prunus armeniaca L.) cultivars native to China were determined by the S-allele PCR approach and the results were confirmed by cross-pollination tests among these cultivars. Primer combination EM-PC2consFD + EM-PC3consR,
based on the conserved regions C2 and C3 of Rosaceous
S-RNase genes, was the most useful primer combination for identifying Chinese apricot S-alleles. Twelve S-RNase alleles were identified using this primer combination, and they were defined as follows: S
9 was 657 bp, S
10 was 266 bp, S
11 was 464 bp, S
12 was 360 bp, S
13 was 401 bp, S
14 was 492 bp, S
15 was 469 bp, S
16 was 481 bp, S
17 was 487 bp, S
18 was 1337 bp, S
19 was 546 bp and S
20 was 1934 bp. S
11–S
20 were new S-RNase genes deposited in GenBank under accession numbers DQ868316, DQ870628-DQ870634, EF133689 and EF160078, respectively.
Our findings contribute to a more efficient breeding program of Chinese apricot and further studies on the S-RNase genes. |
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Keywords: | Prunus armeniaca Self-incompatibility S-RNase S-genotype |
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