Induction of microspore embryos in a CMS line of Brassica juncea and formation of the androgenic plantlets |
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Authors: | Meghna Rana Malik NS Rangaswamy KR Shivanna |
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Institution: | (1) Department of Botany, University of Delhi, Delhi, 110 007, India;(2) Present address: KC-29B, Ashok Vihar I, Delhi, 110 052, India |
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Abstract: | Anther culture of two wide hybrids (Diplotaxis erucoides × Brassica campestris) × B. juncea and (D. berthautii × B. campestris) × B. juncea, their CMS lines and the parent species elicited a range of responses highlighting the importance of the genotype. Androgenesis
was expressed in cultured anthers of CMS (D. erucoides) B. juncea (22.8%), in restored pollen fertile plants of this CMS line (1.66%), and in the parent, B. juncea cv Pusa Bold (13.02%). AgNO3 was essential for androgenic response in the CMS lines, and it markedly increased the frequency of androgenesis in the cultivated
species. Multiple crops of microspore embryos were obtained from responsive anthers of CMS plants in anther recultures. As
high as92% microspore embryos of the CMS line germinated on basal B5medium and formed normal plantlets.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Brassica juncea CMS line Diplotaxis spp microspore embryos silver nitrate wide hybridization |
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