Inheritance and molecular tagging of MYMIV resistance gene in blackgram (Vigna mungo L. Hepper)

Yellow Mosaic disease (YMD) is one of the most destructive diseases of blackgram (Vigna mungo) causing heavy yield losses every year. Mungbean Yellow Mosaic India Virus (MYMIV) is one of the YMD causing begomoviruses prevalent in the major blackgram growing area (northern and central part) of India. Inheritance of MYMIV resistance gene was studied in blackgram using F₁, F₂ and F_2:3 derived from cross DPU 88-31(resistant)× AKU 9904 (susceptible). The results of genetic analysis showed that a single dominant gene controls the MYMIV resistance in blackgram genotype DPU 88-31. The F₂ population from the same cross was also used to tag and map the MYMIV resistance gene using SSR markers. Out of 361 markers, 31 were found polymorphic between the parents. However, marker CEDG 180 was found to be linked with resistance gene following the bulked segregant analysis. This marker was mapped in the F₂ mapping population of 168 individuals at a map distance of 12.9 cm. The validation of this marker in nine resistant and seven susceptible genotypes has suggested its use in marker assisted breeding for developing MYMIV resistant genotypes in blackgram.