| 黑核桃叶片基因组DNA提取方法比较研究 |
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| 引用本文： | 张丽,吴全忠,黄学琴,李苗,陈虞超,刘立武,宋玉霞.黑核桃叶片基因组DNA提取方法比较研究[J].中国农学通报,2011,27(28):125-129. |
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| 作者姓名： | 张丽 吴全忠 黄学琴 李苗 陈虞超 刘立武 宋玉霞 |
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| 作者单位： | 1. 宁夏农业生物技术重点实验室,银川,750002
2. 宁夏中宁轿子山林场,宁夏中宁,755100 |
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| 基金项目： | 宁夏林业科学技术研究项目"美国黑核桃优良品系选育研究及新技术示范推广"，宁夏科技计划资助"宁夏引进美国黑核桃优良品系筛选研究" |
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| 摘 要： | 探索从富含多酚和多糖等次生代谢物质的黑核桃叶片中获得高质量DNA的提取方法。以黑核桃与核桃叶片为材料，采用SDS、CTAB和改良CTAB 3种DNA提取方法对黑核桃与核桃基因组DNA提取效果进行比较研究，并通过琼脂糖凝胶电泳与紫外分光光度计检测所提取样品的质量；SDS与CTAB法重复性较差，提取的黑核桃DNA易降解；改良CTAB法可有效抑制酚类物质及细胞内多糖对DNA纯度的影响，对样品提取的DNA也无降解现象，纯度较高，得到的DNA A260/A280值均处于1.80~1.95之间；改良CTAB法是适用于高质量黑核桃DNA的提取方法。
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| 关 键 词： | 黑核桃 核桃 叶片 DNA提取 |
| 收稿时间： | 2011/3/24 0:00:00 |
| 修稿时间： | 2011/4/22 0:00:00 |
Comparison of DNA Extraction Methods from Leaves of Black Walnuts |
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| Zhang Li,Huang Xueqin,Wu Quanzhong,Li Miao,Chen Yuchao,Liu Liwu,Song Yuxia.Comparison of DNA Extraction Methods from Leaves of Black Walnuts[J].Chinese Agricultural Science Bulletin,2011,27(28):125-129. |
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| Authors: | Zhang Li Huang Xueqin Wu Quanzhong Li Miao Chen Yuchao Liu Liwu Song Yuxia |
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| Institution: | Zhang Li 1,Huang Xueqin 2,Wu Quanzhong 2,Li Miao 1,Chen Yuchao 1,Liu Liwu 2,Song Yuxia 1(1 Ningxia Key Laboratory for Agro Biotechnology,Yinchuan 750002,2 Forest of Ningxia Zhongning Jiaozishan,Zhongning Ningxia 755100) |
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| Abstract: | The aim was to obtain the high quality DNA extracted method from leaves of black walnut which contained polysaccharides and polyphenolic compounds.Using leaves of Black walnut and walnut as experimental materials,the genomic DNA extraction of three methods namely SDS,CTAB and modified CTAB were compared.The DNA samples extracted by three methods were tested by agarose gel electrophoresis,ultraviolet spectrophotometer.The results showed that repeatability of the methods of SDS and CTAB were poor,it was diffi... |
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| Keywords: | black walnut walnut leaves DNA extract |
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